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Recombinant human VEGF 165A protein (Active) is a Human Full Length protein, in the 27 to 191 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

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Images

Functional Studies - Recombinant human VEGF 165A protein (Active) (AB259412), expandable thumbnail
  • SDS-PAGE - Recombinant human VEGF 165A protein (Active) (AB259412), expandable thumbnail
  • Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412), expandable thumbnail
  • HPLC - Recombinant human VEGF 165A protein (Active) (AB259412), expandable thumbnail

Publications

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biologically active

Yes

Amino acid sequence

A P M A E G G G Q N H H E V V K F M D V Y Q R S Y C H P I E T L V D I F Q E Y P D E I E Y I F K P S C V P L M R C G G C C N D E G L E C V P T E E S N I T M Q I M R I K P H Q G Q H I G E M S F L Q H N K C E C R P K K D R A R Q E N P C G P C S E R R K H L F V Q D P Q T C K C S C K N T D S R C K A R Q L E L N E R T C R C D K P R R

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Cell Culture

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

N-VEGFParticipates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).VEGFAGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437).Isoform VEGF165BBinds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Alternative names

Recommended products

Recombinant human VEGF 165A protein (Active) is a Human Full Length protein, in the 27 to 191 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, MS, HPLC, Cell Culture

Biological activity

Fully biologically active determined by the dose dependent increase in proliferation of Human umbilical vein endothelial cells.

ED50 is ≤ 5.3 ng/mL, corresponding to a specific activity of 0.19 x 106 units/mg.

Accession
P15692-4
Animal free

Yes

Species

Human

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

A P M A E G G G Q N H H E V V K F M D V Y Q R S Y C H P I E T L V D I F Q E Y P D E I E Y I F K P S C V P L M R C G G C C N D E G L E C V P T E E S N I T M Q I M R I K P H Q G Q H I G E M S F L Q H N K C E C R P K K D R A R Q E N P C G P C S E R R K H L F V Q D P Q T C K C S C K N T D S R C K A R Q L E L N E R T C R C D K P R R

Accession

P15692

Protein length

Full Length

Predicted molecular weight

19.22 kDa

Amino acids

27 to 191

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

Purity =95%  by HPLC

General info

Function

N-VEGF

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Post-translational modifications

Vascular endothelial growth factor A, long form

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VEGF 165A or Vascular Endothelial Growth Factor 165A is an important protein that plays a role in angiogenesis which is the growth of new blood vessels. Also known as VEGF-A or VEGF isoform 165 this protein has a mass of around 23 kDa when it is in its biotinylated form. VEGF 165A is expressed in various cell types including endothelial cells which form the lining of blood vessels as well as in smooth muscle cells and certain tumor cells. The protein is often targeted in research using anti-VEGF antibodies.

Biological function summary

This protein acts directly on endothelial cells to stimulate their proliferation and migration. VEGF 165A is part of a larger family of VEGF proteins that share similar structures and functions. It plays a critical role in both physiological processes like wound healing and pathological conditions like cancer where its expression becomes dysregulated. The protein can bind to receptors on the cell surface initiating cell signaling pathways that lead to angiogenesis.

Pathways

VEGF 165A functions prominently in the VEGF signaling pathway and the MAPK/ERK pathway. Through these pathways it interacts with other proteins such as VEGF receptors particularly VEGFR-2 to propagate signals leading to angiogenesis and increased vascular permeability. These pathways are vital in ensuring that tissues receive an appropriate blood supply under various conditions including growth and repair processes.

Associated diseases and disorders

VEGF 165A shows an association with cancer and age-related macular degeneration (AMD). Overexpression of VEGF 165A is linked to tumor growth because it helps form the blood vessels that supply the tumor known as tumor angiogenesis. Similarly in AMD abnormal blood vessel growth driven by VEGF 165A leads to damage in the retina. Anti-VEGF therapies often target this protein to treat these conditions by inhibiting its action on blood vessel formation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Recombinant human VEGF 165A protein (Active) (ab259412), expandable thumbnail

    Functional Studies - Recombinant human VEGF 165A protein (Active) (ab259412)

    Fully biologically active determined by the dose dependent increase in proliferation of Human umbilical vein endothelial cells.

    ED50 is ≤ 5.3 ng/mL, corresponding to a specific activity of 0.19 x 106 units/mg.

    Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

    Lot: ​GR3373539-2

  • SDS-PAGE - Recombinant human VEGF 165A protein (Active) (ab259412), expandable thumbnail

    SDS-PAGE - Recombinant human VEGF 165A protein (Active) (ab259412)

    SDS-PAGE analysis of ab259412.

    Protein migrates higher than expected due to glycosylation.

  • Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (ab259412), expandable thumbnail

    Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (ab259412)

    Mass determination by ESI TOF.

    Predicted mass is 19223.56 (+/- 10 Da by ESI-TOF). Mass at 19066.94 is due to loss of arginine on the C-terminus; peak at 19099.52 is a process related impurity.

  • HPLC - Recombinant human VEGF 165A protein (Active) (ab259412), expandable thumbnail

    HPLC - Recombinant human VEGF 165A protein (Active) (ab259412)

    HPLC analysis of ab259412.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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