Recombinant human VEGF Receptor 2 protein (Active) is a Human Fragment protein, in the 789 to 1356 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>70% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
V K R A N G G E L K T G Y L S I V M D P D E L P L D E H C E R L P Y D A S K W E F P R D R L K L G K P L G R G A F G Q V I E A D A F G I D K T A T C R T V A V K M L K E G A T H S E H R A L M S E L K I L I H I G H H L N V V N L L G A C T K P G G P L M V I V E F C K F G N L S T Y L R S K R N E F V P Y K T K G A R F R Q G K D Y V G A I P V D L K R R L D S I T S S Q S S A S S G F V E E K S L S D V E E E E A P E D L Y K D F L T L E H L I C Y S F Q V A K G M E F L A S R K C I H R D L A A R N I L L S E K N V V K I C D F G L A R D I Y K D P D Y V R K G D A R L P L K W M A P E T I F D R V Y T I Q S D V W S F G V L L W E I F S L G A S P Y P G V K I D E E F C R R L K E G T R M R A P D Y T T P E M Y Q T M L D C W H G E P S Q R P T F S E L V E H L G N L L Q A N A Q Q D G K D Y I V L P I S E T L S M E E D S G L S L P T S P V S C M E E E E V C D P K F H Y D N T A G I S Q Y L Q N S K R K S R P V S V K T F E D I P L E E P E V K V I P D D N Q T D S G M V L A S E E L K T L E D R T K L S P S F G G M V P S K S R E S V A S E G S N Q T S G Y Q S G Y H S D D T D T T V Y S S E E A E L L K L I E I G V Q T G S T A Q I L Q P D S G T T L S S P P V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1, KDR, KDR, VEGFR2, FLK1
Recommended products
Recombinant human VEGF Receptor 2 protein (Active) is a Human Fragment protein, in the 789 to 1356 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Alternative names
Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1, KDR, KDR, VEGFR2, FLK1
Key facts
- Purity
>70% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
The specific activity of ab268703 was determined to be 70 nmol/min/mg in a kinase assay using MBP substrate.
- Accession
- P35968-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
V K R A N G G E L K T G Y L S I V M D P D E L P L D E H C E R L P Y D A S K W E F P R D R L K L G K P L G R G A F G Q V I E A D A F G I D K T A T C R T V A V K M L K E G A T H S E H R A L M S E L K I L I H I G H H L N V V N L L G A C T K P G G P L M V I V E F C K F G N L S T Y L R S K R N E F V P Y K T K G A R F R Q G K D Y V G A I P V D L K R R L D S I T S S Q S S A S S G F V E E K S L S D V E E E E A P E D L Y K D F L T L E H L I C Y S F Q V A K G M E F L A S R K C I H R D L A A R N I L L S E K N V V K I C D F G L A R D I Y K D P D Y V R K G D A R L P L K W M A P E T I F D R V Y T I Q S D V W S F G V L L W E I F S L G A S P Y P G V K I D E E F C R R L K E G T R M R A P D Y T T P E M Y Q T M L D C W H G E P S Q R P T F S E L V E H L G N L L Q A N A Q Q D G K D Y I V L P I S E T L S M E E D S G L S L P T S P V S C M E E E E V C D P K F H Y D N T A G I S Q Y L Q N S K R K S R P V S V K T F E D I P L E E P E V K V I P D D N Q T D S G M V L A S E E L K T L E D R T K L S P S F G G M V P S K S R E S V A S E G S N Q T S G Y Q S G Y H S D D T D T T V Y S S E E A E L L K L I E I G V Q T G S T A Q I L Q P D S G T T L S S P P V
- Accession
- Protein length
Fragment
- Amino acids
789 to 1356
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- Activity summary
VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
- Biological function summary
VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.
- Pathways
VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.
- Associated diseases and disorders
VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
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2 product images
Functional Studies - Recombinant human VEGF Receptor 2 protein (Active) (ab268703)
The specific activity of ab268703 was determined to be 70 nmol/min/mg in a kinase assay using MBP substrate.
SDS-PAGE - Recombinant human VEGF Receptor 2 protein (Active) (ab268703)
SDS-PAGE analysis of ab268703.
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