JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB55566

Recombinant Human VEGFA protein

5

(1 Review)

|

(6 Publications)

Recombinant Human VEGFA protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images
SDS-PAGE - Recombinant Human VEGFA protein (AB55566)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human VEGFA protein (AB55566)

ab55566 on SDS-PAGE

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P15692

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.242% Tris

storage-buffer

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB46154

Anti-VEGFA antibody

Lab Essentials

Western blot - Anti-VEGFA antibody (AB46154)

  • 5
  • 29
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Assay Kits

AB222510

Human VEGF ELISA Kit

SimpleStep

ELISA - Human VEGF ELISA Kit (AB222510)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab55566 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/vegfa-antibody-mabcam-68334-ab68334'>ab68334</a>.</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"23.6 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P15692","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
style
left-column

Specifications

Form

Liquid

Additional notes

Proprietary purification method

style
left-column

General info

Function

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Post-translational modifications

Vascular endothelial growth factor A, long form. Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.

style
left-column

Product protocols

style
left-column

Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 13: PubMed38004368

2023

Mathematical Modeling of Non-Small-Cell Lung Cancer Biology through the Experimental Data on Cell Composition and Growth of Patient-Derived Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Rushan Sulimanov,Konstantin Koshelev,Vladimir Makarov,Alexandre Mezentsev,Mikhail Durymanov,Lilian Ismail,Komal Zahid,Yegor Rumyantsev,Ilya Laskov

Cell death & disease 11:1016 PubMed33247124

2020

Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Aisong Guo,Yuanyuan Tu,Wendie Li,Lele Li,Wangrui Liu,Yuanyuan Ju,Yamei Zhou,Aimin Sang,Manhui Zhu

Journal of medicinal food : PubMed32614624

2020

Angiogenesis is Differentially Modulated by Anthocyanin and Phenolic Acid Extracts from Wild Blueberry () Through PI3K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Panagiotis Tsakiroglou,James Weber,Sharon Ashworth,Cristian Del Bo',Dorothy Klimis-Zacas

Cell death & disease 9:906 PubMed30185783

2018

Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xiao,Weilong Zhong,Jianmin Zhao,Baoxin Qian,Huijuan Liu,Shuang Chen,Kailiang Qiao,Yueyang Lei,Shumin Zong,Hongzhi Wang,Yuan Liang,Heng Zhang,Jing Meng,Honggang Zhou,Tao Sun,Yanrong Liu,Cheng Yang

PloS one 10:e0144823 PubMed26657744

2015

Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium.

Applications

WB

Species

Unspecified reactive species

Robert K Hunter,Chris D Nevitt,Jeremy T Gaskins,Bradley B Keller,Henry C L Bohler,Amanda J LeBlanc

Cardiovascular research 93:111-9 PubMed22002459

2011

ARNO regulates VEGF-dependent tissue responses by stabilizing endothelial VEGFR-2 surface expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna K Mannell,Joachim Pircher,Daniel I Chaudhry,Stefan K C Alig,Elisabeth G Koch,Ramona Mettler,Ulrich Pohl,Florian Krötz
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com