Recombinant human VEGFA protein (Active) is a Human Fragment protein, in the 27 to 147 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- ELISA, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
A P M A E G G G Q N H H E V V K F M D V Y Q R S Y C H P I E T L V D I F Q E Y P D E I E Y I F K P S C V P L M R C G G C C N D E G L E C V P T E E S N I T M Q I M R I K P H Q G Q H I G E M S F L Q H N K C E C R P K K D R A R Q E K C D K P R R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The protein has a MW of 14.1 kDa. As a result of glycosylation, the protein migrates as 19 kDa and 20 kDa under reducing condition, and 40-45 kDa (homodimer) under non-reducing condition (SDS-PAGE). |
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Target data
Function
N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity). VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437). Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Alternative names
VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF
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Recombinant human VEGFA protein (Active) is a Human Fragment protein, in the 27 to 147 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- ELISA, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. ab155722 at 2 μg/mL can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL.
- Accession
- P15692-12
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- A P M A E G G G Q N H H E V V K F M D V Y Q R S Y C H P I E T L V D I F Q E Y P D E I E Y I F K P S C V P L M R C G G C C N D E G L E C V P T E E S N I T M Q I M R I K P H Q G Q H I G E M S F L Q H N K C E C R P K K D R A R Q E K C D K P R R
- Accession
- P15692
- Protein length
- Fragment
- Predicted molecular weight
- 14.1 kDa
- Amino acids
- 27 to 147
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
ab155722 was lyophilized from 0.22 µm filtered solution.
General info
- Function
N-VEGF
- Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
- Post-translational modifications
Vascular endothelial growth factor A, long form
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
- Biological function summary
VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.
- Pathways
Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.
- Associated diseases and disorders
VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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2 product images
Functional Studies - Recombinant human VEGFA protein (Active) (ab155722)
SDS-PAGE of ab155722 under reducing (R) and non-reducing (NR) conditions, stained overnight with Coomassie Blue. The protein migrates as 19 kDa and 20 kDa under reducing conditions, and 40-45 kDa (homodimer) under non-reducing conditions.
Functional Studies - Recombinant human VEGFA protein (Active) (ab155722)
ab155722 at 2 μg/mL can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL.
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