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AB155739

Recombinant human VEGFC protein

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(1 Publication)

Recombinant human VEGFC protein is a Human Full Length protein, in the 103 to 227 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Vascular endothelial growth factor C, VEGF-C, Flt4 ligand, Vascular endothelial growth factor-related protein, Flt4-L, VRP, VEGFC

3 Images
Functional Studies - Recombinant human VEGFC protein (AB155739)
  • FuncS

Unknown

Functional Studies - Recombinant human VEGFC protein (AB155739)

Immobilized Human VEGF-C, His Tag at 2 µg/mL (100 µL/well) can bind Biotinylated Human VEGF R1, His,Avitag with a linear range of 0.02-0.625 µg/mL.

Functional Studies - Recombinant human VEGFC protein (AB155739)
  • FuncS

Unknown

Functional Studies - Recombinant human VEGFC protein (AB155739)

Immobilized Human VEGF R3, Fc Tag at 5 µg/mL (100 µL/well) can bind Human VEGF-C, His Tag with a linear range of 2-20 ng/mL.

SDS-PAGE - Recombinant human VEGFC protein (AB155739)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human VEGFC protein (AB155739)

SDS-PAGE analysis of reduced ab155739 stained overnight with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Immobilized Human VEGF R3, Fc Tag at 5 μg/mL (100 μL/well) can bind Human VEGF-C, His Tag with a linear range of 2-20 ng/mL.

Accession

P49767

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 250 µg/ml.

Storage buffer

pH: 7.4 Constituents: PBS, 5% Trehalose

storage-buffer

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Primary Antibodies

AB303710

Anti-VEGFC antibody [EPR22854-144] - BSA and Azide free (Capture)

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Primary Antibodies

AB46154

Anti-VEGFA antibody

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Western blot - Anti-VEGFA antibody (AB46154)

  • 5
  • 29
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>As a result of glycosylation, DTT-reduced ab155739 migrates as a 19-25 kDa polypeptide in SDS-PAGE.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"TEETIKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRR","proteinLength":"Full Length","predictedMolecularWeight":"15 kDa","actualMolecularWeight":null,"aminoAcidEnd":227,"aminoAcidStart":103,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P49767","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGFC also known as Vascular Endothelial Growth Factor C is a member of the VEGF family involved in angiogenesis and lymphangiogenesis. It has a molecular mass of approximately 35 kDa. VEGFC is expressed in various tissues with high levels in the heart lung and muscle. It acts primarily through its interaction with the VEGFR-3 receptor stimulating the formation of blood and lymphatic vessels. This protein plays an important role in the development and maintenance of the circulatory and lymphatic systems.
Biological function summary

VEGFC contributes to the regulation of vascular and lymphatic endothelial cell functions. It is not known to be part of a larger protein complex but exerts its functions through interactions with receptors such as VEGFR-3. By promoting cell proliferation and migration VEGFC assists in the repair and regeneration of tissues. Its role in fluid balance is essential as it helps maintain tissue equilibrium and immune function.

Pathways

VEGFC is integral to the VEGF signaling and lymphatic vessel development pathways. Within these processes VEGFC works alongside other proteins such as VEGFA and VEGFB to modulate vascular permeability and vessel formation. The interaction between VEGFC and the VEGFR-3 receptor specifically mediates lymphatic endothelial cell activation and function linking it to vital physiological processes like inflammation and tissue repair.

VEGFC is associated with cancer and lymphedema. Its role in promoting angiogenesis is critical in tumor growth and metastasis with its overexpression often linked to cancer progression. Furthermore insufficient VEGFC activity contributes to the development of lymphedema due to inadequate lymphatic drainage. In cancer pathology VEGFC works together with VEGFR-3 contributing to the abnormal growth of lymphatic vessels which in turn facilitates cancer cell dissemination.
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Specifications

Form

Lyophilized

Additional notes

Purified by Immobilized metal affinity chromatography.

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General info

Function

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Post-translational modifications

Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.

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Product protocols

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Target data

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors.
See full target information VEGFC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34299365

2021

Extracellular Hsp90α Promotes Tumor Lymphangiogenesis and Lymph Node Metastasis in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Hou,Shuohua Chen,Qi An,Boya Li,Yan Fu,Yongzhang Luo
View all publications
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