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AB289731

Recombinant Human VHL Protein

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Recombinant Human VHL Protein is a Human Full Length protein, in the 1 to 213 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL

2 Images
HPLC - Recombinant Human VHL Protein (AB289731)
  • HPLC

Supplier Data

HPLC - Recombinant Human VHL Protein (AB289731)

HPLC analysis of ab289731

SDS-PAGE - Recombinant Human VHL Protein (AB289731)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human VHL Protein (AB289731)

SDS-PAGE analysis of ab289731

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P40337

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD","proteinLength":"Full Length","predictedMolecularWeight":"24.21 kDa","actualMolecularWeight":null,"aminoAcidEnd":213,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P40337","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Specifications

Form

Lyophilized

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General info

Function

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).

Sequence similarities

Belongs to the VHL family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).
See full target information VHL
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