Recombinant Human Vimentin protein (Tagged-His Tag) is a Human Full Length protein, in the 1 to 466 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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Publications
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- The Journal of biological chemistry 289:6960-82014Identification and characterization of an oocyte factor required for porcine nuclear reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingran Kong et. al.PubMed 24474691
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
Amino acid sequence
M S T R S V S S S S Y R R M F G G P G T A S R P S S S R S Y V T T S T R T Y S L G S A L R P S T S R S L Y A S S P G G V Y A T R S S A V R L R S S V P G V R L L Q D S V D F S L A D A I N T E F K N T R T N E K V E L Q E L N D R F A N Y I D K V R F L E Q Q N K I L L A E L E Q L K G Q G K S R L G D L Y E E E M R E L R R Q V D Q L T N D K A R V E V E R D N L A E D I M R L R E K L Q E E M L Q R E E A E N T L Q S F R Q D V D N A S L A R L D L E R K V E S L Q E E I A F L K K L H E E E I Q E L Q A Q I Q E Q H V Q I D V D V S K P D L T A A L R D V R Q Q Y E S V A A K N L Q E A E E W Y K S K F A D L S E A A N R N N D A L R Q A K Q E S T E Y R R Q V Q S L T C E V D A L K G T N E S L E R Q M R E M E E N F A V E A A N Y Q D T I G R L Q D E I Q N M K E E M A R H L R E Y Q D L L N V K M A L D I E I A T Y R K L L E G E E S R I S L P L P N F S S L N L R E T N L D S L P L V D T H S K R T L L I K T V E T R D G Q V I N E T S Q H H D D L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Alternative names
Vimentin, VIM
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Recombinant Human Vimentin protein (Tagged-His Tag) is a Human Full Length protein, in the 1 to 466 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
- Accession
- P08670-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
M S T R S V S S S S Y R R M F G G P G T A S R P S S S R S Y V T T S T R T Y S L G S A L R P S T S R S L Y A S S P G G V Y A T R S S A V R L R S S V P G V R L L Q D S V D F S L A D A I N T E F K N T R T N E K V E L Q E L N D R F A N Y I D K V R F L E Q Q N K I L L A E L E Q L K G Q G K S R L G D L Y E E E M R E L R R Q V D Q L T N D K A R V E V E R D N L A E D I M R L R E K L Q E E M L Q R E E A E N T L Q S F R Q D V D N A S L A R L D L E R K V E S L Q E E I A F L K K L H E E E I Q E L Q A Q I Q E Q H V Q I D V D V S K P D L T A A L R D V R Q Q Y E S V A A K N L Q E A E E W Y K S K F A D L S E A A N R N N D A L R Q A K Q E S T E Y R R Q V Q S L T C E V D A L K G T N E S L E R Q M R E M E E N F A V E A A N Y Q D T I G R L Q D E I Q N M K E E M A R H L R E Y Q D L L N V K M A L D I E I A T Y R K L L E G E E S R I S L P L P N F S S L N L R E T N L D S L P L V D T H S K R T L L I K T V E T R D G Q V I N E T S Q H H D D L E
- Accession
- P08670
- Protein length
Full Length
- Predicted molecular weight
60 kDa
- Amino acids
1 to 466
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Purity >95% as determined by densitometry.
General info
- Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
- Sequence similarities
Belongs to the intermediate filament family.
- Post-translational modifications
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480). Phosphorylated by STK33 (PubMed:18811945). Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
- Subcellular localisation
Cytoskeleton, Nucleus matrix
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Vimentin protein (Tagged-His Tag) (ab84704)
SDS-PAGE of ab84704 using 3ug Vimentin protein. Detected molecular weight approximately 60 kDa.
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