Recombinant Human Vimentin protein (Tagged-His Tag) is a Human Full Length protein, in the 1 to 466 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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Publications
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- The Journal of biological chemistry 289:6960-82014Identification and characterization of an oocyte factor required for porcine nuclear reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingran Kong et. al.PubMed 24474691
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M S T R S V S S S S Y R R M F G G P G T A S R P S S S R S Y V T T S T R T Y S L G S A L R P S T S R S L Y A S S P G G V Y A T R S S A V R L R S S V P G V R L L Q D S V D F S L A D A I N T E F K N T R T N E K V E L Q E L N D R F A N Y I D K V R F L E Q Q N K I L L A E L E Q L K G Q G K S R L G D L Y E E E M R E L R R Q V D Q L T N D K A R V E V E R D N L A E D I M R L R E K L Q E E M L Q R E E A E N T L Q S F R Q D V D N A S L A R L D L E R K V E S L Q E E I A F L K K L H E E E I Q E L Q A Q I Q E Q H V Q I D V D V S K P D L T A A L R D V R Q Q Y E S V A A K N L Q E A E E W Y K S K F A D L S E A A N R N N D A L R Q A K Q E S T E Y R R Q V Q S L T C E V D A L K G T N E S L E R Q M R E M E E N F A V E A A N Y Q D T I G R L Q D E I Q N M K E E M A R H L R E Y Q D L L N V K M A L D I E I A T Y R K L L E G E E S R I S L P L P N F S S L N L R E T N L D S L P L V D T H S K R T L L I K T V E T R D G Q V I N E T S Q H H D D L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Vimentin, VIM
Recommended products
Recombinant Human Vimentin protein (Tagged-His Tag) is a Human Full Length protein, in the 1 to 466 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P08670-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M S T R S V S S S S Y R R M F G G P G T A S R P S S S R S Y V T T S T R T Y S L G S A L R P S T S R S L Y A S S P G G V Y A T R S S A V R L R S S V P G V R L L Q D S V D F S L A D A I N T E F K N T R T N E K V E L Q E L N D R F A N Y I D K V R F L E Q Q N K I L L A E L E Q L K G Q G K S R L G D L Y E E E M R E L R R Q V D Q L T N D K A R V E V E R D N L A E D I M R L R E K L Q E E M L Q R E E A E N T L Q S F R Q D V D N A S L A R L D L E R K V E S L Q E E I A F L K K L H E E E I Q E L Q A Q I Q E Q H V Q I D V D V S K P D L T A A L R D V R Q Q Y E S V A A K N L Q E A E E W Y K S K F A D L S E A A N R N N D A L R Q A K Q E S T E Y R R Q V Q S L T C E V D A L K G T N E S L E R Q M R E M E E N F A V E A A N Y Q D T I G R L Q D E I Q N M K E E M A R H L R E Y Q D L L N V K M A L D I E I A T Y R K L L E G E E S R I S L P L P N F S S L N L R E T N L D S L P L V D T H S K R T L L I K T V E T R D G Q V I N E T S Q H H D D L E
- Accession
- P08670
- Protein length
- Full Length
- Predicted molecular weight
- 60 kDa
- Amino acids
- 1 to 466
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity >95% as determined by densitometry.
General info
- Function
Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the intermediate filament family.
- Post-translational modifications
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480). Phosphorylated by STK33 (PubMed:18811945). Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
- Subcellular localisation
- Cytoskeleton, Nucleus matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Vimentin protein (Tagged-His Tag) (ab84704)
SDS-PAGE of ab84704 using 3ug Vimentin protein. Detected molecular weight approximately 60 kDa.
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