Recombinant Human Vinculin protein (His tag) is a Human Fragment protein, in the 1 to 1066 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
M P V F H T R T I E S I L E P V A Q Q I S H L V I M H E E G E V D G K A I P D L T A P V A A V Q A A V S N L V R V G K E T V Q T T E D Q I L K R D M P P A F I K V E N A C T K L V Q A A Q M L Q S D P Y S V P A R D Y L I D G S R G I L S G T S D L L L T F D E A E V R K I I R V C K G I L E Y L T V A E V V E T M E D L V T Y T K N L G P G M T K M A K M I D E R Q Q E L T H Q E H R V M L V N S M N T V K E L L P V L I S A M K I F V T T K N S K N Q G I E E A L K N R N F T V E K M S A E I N E I I R V L Q L T S W D E D A W A S K D T E A M K R A L A S I D S K L N Q A K G W L R D P S A S P G D A G E Q A I R Q I L D E A G K V G E L C A G K E R R E I L G T C K M L G Q M T D Q V A D L R A R G Q G S S P V A M Q K A Q Q V S Q G L D V L T A K V E N A A R K L E A M T N S K Q S I A K K I D A A Q N W L A D P N G G P E G E E Q I R G A L A E A R K I A E L C D D P K E R D D I L R S L G E I S A L T S K L A D L R R Q G K G D S P E A R A L A K Q V A T A L Q N L Q T K T N R A V A N S R P A K A A V H L E G K I E Q A Q R W I D N P T V D D R G V G Q A A I R G L V A E G H R L A N V M M G P Y R Q D L L A K C D R V D Q L T A Q L A D L A A R G E G E S P Q A R A L A S Q L Q D S L K D L K A R M Q E A M T Q E V S D V F S D T T T P I K L L A V A A T A P P D A P N R E E V F D E R A A N F E N H S G K L G A T A E K A A A V G T A N K S T V E G I Q A S V K T A R E L T P Q V V S A A R I L L R N P G N Q A A Y E H F E T M K N Q W I D N V E K M T G L V D E A I D T K S L L D A S E E A I K K D L D K C K V A M A N I Q P Q M L V A G A T S I A R R A N R I L L V A K R E V E N S E D P K F R E A V K A A S D E L S K T I S P M V M D A K A V A G N I S D P G L Q K S F L D S G Y R I L G A V A K V R E A F Q P Q E P D F P P P P P D L E Q L R L T D E L A P P K P P L P E G E V P P P R P P P P E E K D E E F P E Q K A G E V I N Q P M M M A A R Q L H D E A R K W S S K G N D I I A A A K R M A L L M A E M S R L V R G G S G T K R A L I Q C A K D I A K A S D E V T R L A K E V A K Q C T D K R I R T N L L Q V C E R I P T I S T Q L K I L S T V K A T M L G R T N I S D E E S E Q A T E M L V H N A Q N L M Q S V K E T V R E A E A A S I K I R T D A G F T L R W V R K T P W Y Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Alternative names
Vinculin, Metavinculin, MV, VCL
Recommended products
Recombinant Human Vinculin protein (His tag) is a Human Fragment protein, in the 1 to 1066 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Immobilized ab276192 at 1 μg/mL (100 μL/well) can bind Vinculin Antibody Rabbit PAb Antigen, the EC50 is 1.0-3.0 ng/mL.
- Accession
- P18206-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M P V F H T R T I E S I L E P V A Q Q I S H L V I M H E E G E V D G K A I P D L T A P V A A V Q A A V S N L V R V G K E T V Q T T E D Q I L K R D M P P A F I K V E N A C T K L V Q A A Q M L Q S D P Y S V P A R D Y L I D G S R G I L S G T S D L L L T F D E A E V R K I I R V C K G I L E Y L T V A E V V E T M E D L V T Y T K N L G P G M T K M A K M I D E R Q Q E L T H Q E H R V M L V N S M N T V K E L L P V L I S A M K I F V T T K N S K N Q G I E E A L K N R N F T V E K M S A E I N E I I R V L Q L T S W D E D A W A S K D T E A M K R A L A S I D S K L N Q A K G W L R D P S A S P G D A G E Q A I R Q I L D E A G K V G E L C A G K E R R E I L G T C K M L G Q M T D Q V A D L R A R G Q G S S P V A M Q K A Q Q V S Q G L D V L T A K V E N A A R K L E A M T N S K Q S I A K K I D A A Q N W L A D P N G G P E G E E Q I R G A L A E A R K I A E L C D D P K E R D D I L R S L G E I S A L T S K L A D L R R Q G K G D S P E A R A L A K Q V A T A L Q N L Q T K T N R A V A N S R P A K A A V H L E G K I E Q A Q R W I D N P T V D D R G V G Q A A I R G L V A E G H R L A N V M M G P Y R Q D L L A K C D R V D Q L T A Q L A D L A A R G E G E S P Q A R A L A S Q L Q D S L K D L K A R M Q E A M T Q E V S D V F S D T T T P I K L L A V A A T A P P D A P N R E E V F D E R A A N F E N H S G K L G A T A E K A A A V G T A N K S T V E G I Q A S V K T A R E L T P Q V V S A A R I L L R N P G N Q A A Y E H F E T M K N Q W I D N V E K M T G L V D E A I D T K S L L D A S E E A I K K D L D K C K V A M A N I Q P Q M L V A G A T S I A R R A N R I L L V A K R E V E N S E D P K F R E A V K A A S D E L S K T I S P M V M D A K A V A G N I S D P G L Q K S F L D S G Y R I L G A V A K V R E A F Q P Q E P D F P P P P P D L E Q L R L T D E L A P P K P P L P E G E V P P P R P P P P E E K D E E F P E Q K A G E V I N Q P M M M A A R Q L H D E A R K W S S K G N D I I A A A K R M A L L M A E M S R L V R G G S G T K R A L I Q C A K D I A K A S D E V T R L A K E V A K Q C T D K R I R T N L L Q V C E R I P T I S T Q L K I L S T V K A T M L G R T N I S D E E S E Q A T E M L V H N A Q N L M Q S V K E T V R E A E A A S I K I R T D A G F T L R W V R K T P W Y Q
- Accession
- P18206
- Protein length
- Fragment
- Predicted molecular weight
- 118 kDa
- Amino acids
- 1 to 1066
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
- Sequence similarities
Belongs to the vinculin/alpha-catenin family.
- Post-translational modifications
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Vinculin protein (His tag) (ab276192)
SDS-PAGE analysis of ab276192.
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