Recombinant Human Vinculin protein (Tagged) is a Human Fragment protein, in the 2 to 235 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
P V F H T R T I E S I L E P V A Q Q I S H L V I M H E E G E V D G K A I P D L T A P V A A V Q A A V S N L V R V G K E T V Q T T E D Q I L K R D M P P A F I K V E N A C T K L V Q A A Q M L Q S D P Y S V P A R D Y L I D G S R G I L S G T S D L L L T F D E A E V R K I I R V C K G I L E Y L T V A E V V E T M E D L V T Y T K N L G P G M T K M A K M I D E R Q Q E L T H Q E H R V M L V N S M N T V K E L L P V L I S A M K I F V T T K N S K N Q G I E E A L K N R N F T V E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Alternative names
Vinculin, Metavinculin, MV, VCL
Recommended products
Recombinant Human Vinculin protein (Tagged) is a Human Fragment protein, in the 2 to 235 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P18206-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- P V F H T R T I E S I L E P V A Q Q I S H L V I M H E E G E V D G K A I P D L T A P V A A V Q A A V S N L V R V G K E T V Q T T E D Q I L K R D M P P A F I K V E N A C T K L V Q A A Q M L Q S D P Y S V P A R D Y L I D G S R G I L S G T S D L L L T F D E A E V R K I I R V C K G I L E Y L T V A E V V E T M E D L V T Y T K N L G P G M T K M A K M I D E R Q Q E L T H Q E H R V M L V N S M N T V K E L L P V L I S A M K I F V T T K N S K N Q G I E E A L K N R N F T V E
- Accession
- P18206
- Protein length
- Fragment
- Predicted molecular weight
- 53 kDa
- Amino acids
- 2 to 235
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
- Sequence similarities
Belongs to the vinculin/alpha-catenin family.
- Post-translational modifications
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human Vinculin protein (Tagged) (ab238349)
ab238349 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product protocols
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