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AB179498

Recombinant human Visfatin protein

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Recombinant human Visfatin protein is a Human Fragment protein, in the 27 to 491 aa range, expressed in Escherichia coli, with >90%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor

1 Images
SDS-PAGE - Recombinant human Visfatin protein (AB179498)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Visfatin protein (AB179498)

SDS PAGE analysis of ab179498 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from Human PBMCs at 100 ng/mL.

Accession

P43490

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in 20 mM HCl

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH","proteinLength":"Fragment","predictedMolecularWeight":"52.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":491,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P43490","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
True
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Specifications

Form

Lyophilized

Additional notes

Determined by Reducing and Non-reducing SDS-PAGE.

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General info

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

Sequence similarities

Belongs to the NAPRTase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
See full target information NAMPT
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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB236874

Anti-Visfatin antibody [EPR21980]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (AB236874)

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Assay Kits

AB264623

Human Visfatin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)

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Assay Kits

AB221819

NAMPT Activity Assay Kit (Colorimetric)

Functional Studies - NAMPT Activity Assay Kit (Colorimetric) (AB221819)

  • 0
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