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AB198090

Recombinant human Visfatin protein (Tag Free)

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(2 Publications)

Recombinant human Visfatin protein (Tag Free) is a Human Full Length protein, in the 2 to 491 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor

2 Images
Functional Studies - Recombinant human Visfatin protein (Tag Free) (AB198090)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Visfatin protein (Tag Free) (AB198090)

Activity assay using ab198090.

SDS-PAGE - Recombinant human Visfatin protein (Tag Free) (AB198090)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Visfatin protein (Tag Free) (AB198090)

4-20% SDS-PAGE analysis of 2 μg of ab198090 with Coomassie staining.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 2 pmol/min/μg.

Assay conditions: The 50 μl reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 μg/ml of alcohol dehydrogenase, 10 μg/mL of NMNAT, 1.5% alcohol, 1 mM DTT, 0.02% BSA. Reactions were incubated at room temperature for 30 min, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured.

Accession

P43490

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB236874

Anti-Visfatin antibody [EPR21980]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (AB236874)

  • 0
  • 0
Assay Kits

AB264623

Human Visfatin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)

  • 0
  • 0
Assay Kits

AB221819

NAMPT Activity Assay Kit (Colorimetric)

Functional Studies - NAMPT Activity Assay Kit (Colorimetric) (AB221819)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"GPLGSNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH","proteinLength":"Full Length","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":491,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P43490","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

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General info

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

Sequence similarities

Belongs to the NAPRTase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
See full target information NAMPT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 15: PubMed37242714

2023

Iminosugar-Based Nicotinamide Phosphoribosyltransferase (NAMPT) Inhibitors as Potential Anti-Pancreatic Cancer Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Conforti,Andrea Benzi,Irene Caffa,Santina Bruzzone,Alessio Nencioni,Alberto Marra

European journal of medicinal chemistry 250:115170 PubMed36787658

2023

Synthesis and structure-activity relationship of new nicotinamide phosphoribosyltransferase inhibitors with antitumor activity on solid and haematological cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Fratta,Paulina Biniecka,Antonio J Moreno-Vargas,Ana T Carmona,Aimable Nahimana,Michel A Duchosal,Francesco Piacente,Santina Bruzzone,Irene Caffa,Alessio Nencioni,Inmaculada Robina
View all publications
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