Recombinant human Visfatin protein is a Human Full Length protein, in the 2 to 491 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE, FuncS.
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- Pharmaceutics 15:2023Iminosugar-Based Nicotinamide Phosphoribosyltransferase (NAMPT) Inhibitors as Potential Anti-Pancreatic Cancer Agents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Conforti,Andrea Benzi,Irene Caffa,Santina Bruzzone,Alessio Nencioni,Alberto MarraPubMed 37242714
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
G P L G S N P A A E A E F N I L L A T D S Y K V T H Y K Q Y P P N T S K V Y S Y F E C R E K K T E N S K L R K V K Y E E T V F Y G L Q Y I L N K Y L K G K V V T K E K I Q E A K D V Y K E H F Q D D V F N E K G W N Y I L E K Y D G H L P I E I K A V P E G F V I P R G N V L F T V E N T D P E C Y W L T N W I E T I L V Q S W Y P I T V A T N S R E Q K K I L A K Y L L E T S G N L D G L E Y K L H D F G Y R G V S S Q E T A G I G A S A H L V N F K G T D T V A G L A L I K K Y Y G T K D P V P G Y S V P A A E H S T I T A W G K D H E K D A F E H I V T Q F S S V P V S V V S D S Y D I Y N A C E K I W G E D L R H L I V S R S T Q A P L I I R P D S G N P L D T V L K V L E I L G K K F P V T E N S K G Y K L L P P Y L R V I Q G D G V D I N T L Q E I V E G M K Q K M W S I E N I A F G S G G G L L Q K L T R D L L N C S F K C S Y V V T N G L G I N V F K D P V A D P N K R S K K G R L S L H R T P A G N F V T L E E G K G D L E E Y G Q D L L H T V F K N G K V T K S Y S F D E I R K N A Q L N I E L E A A H H
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
Alternative names
PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor
Recommended products
Recombinant human Visfatin protein is a Human Full Length protein, in the 2 to 491 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 2 pmol/min/μg.
Assay conditions: The 50 μl reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 μg/ml of alcohol dehydrogenase, 10 μg/mL of NMNAT, 1.5% alcohol, 1 mM DTT, 0.02% BSA. Reactions were incubated at room temperature for 30 min, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured. - Accession
- P43490-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- G P L G S N P A A E A E F N I L L A T D S Y K V T H Y K Q Y P P N T S K V Y S Y F E C R E K K T E N S K L R K V K Y E E T V F Y G L Q Y I L N K Y L K G K V V T K E K I Q E A K D V Y K E H F Q D D V F N E K G W N Y I L E K Y D G H L P I E I K A V P E G F V I P R G N V L F T V E N T D P E C Y W L T N W I E T I L V Q S W Y P I T V A T N S R E Q K K I L A K Y L L E T S G N L D G L E Y K L H D F G Y R G V S S Q E T A G I G A S A H L V N F K G T D T V A G L A L I K K Y Y G T K D P V P G Y S V P A A E H S T I T A W G K D H E K D A F E H I V T Q F S S V P V S V V S D S Y D I Y N A C E K I W G E D L R H L I V S R S T Q A P L I I R P D S G N P L D T V L K V L E I L G K K F P V T E N S K G Y K L L P P Y L R V I Q G D G V D I N T L Q E I V E G M K Q K M W S I E N I A F G S G G G L L Q K L T R D L L N C S F K C S Y V V T N G L G I N V F K D P V A D P N K R S K K G R L S L H R T P A G N F V T L E E G K G D L E E Y G Q D L L H T V F K N G K V T K S Y S F D E I R K N A Q L N I E L E A A H H
- Accession
- P43490
- Protein length
- Full Length
- Predicted molecular weight
- 56 kDa
- Amino acids
- 2 to 491
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
- Sequence similarities
Belongs to the NAPRTase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
Functional Studies - Recombinant human Visfatin protein (ab198090)
Activity assay using ab198090.
SDS-PAGE - Recombinant human Visfatin protein (ab198090)
4-20% SDS-PAGE analysis of 2 μg of ab198090 with Coomassie staining.
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