Recombinant Human Vitamin D Receptor protein is a Human Full Length protein, in the 1 to 427 aa range, expressed in Baculovirus, with >95% purity and suitable for GSA, EMSA, WB.
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Key facts
- Purity
- >95% HPLC
- Expression system
- Baculovirus
- Tags
- Tag free
- Applications
- GSA, EMSA, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application GSA | Reactivity Reacts | Dilution info - | Notes - |
Application EMSA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab82068 can be used as a WB positive control in conjunction with ab82566. |
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Target data
Function
Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed:10678179, PubMed:15728261, PubMed:16913708, PubMed:28698609, PubMed:37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed:28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed:28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed:12016314, PubMed:32354638).
Alternative names
NR1I1, VDR, Vitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1
Recommended products
Recombinant Human Vitamin D Receptor protein is a Human Full Length protein, in the 1 to 427 aa range, expressed in Baculovirus, with >95% purity and suitable for GSA, EMSA, WB.
Key facts
- Purity
- >95% HPLC
- Expression system
- Baculovirus
- Tags
- Tag free
- Applications
- GSA, EMSA, WB
- Biologically active
- No
- Accession
- P11473-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA
Sequence info
Amino acid sequence
- Accession
- P11473
- Protein length
- Full Length
- Amino acids
- 1 to 427
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified to >95% purity.
General info
- Function
Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed:10678179, PubMed:15728261, PubMed:16913708, PubMed:28698609, PubMed:37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed:28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed:28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed:12016314, PubMed:32354638).
- Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
- Post-translational modifications
Ubiquitinated by UBR5, leading to its degradation: UBR5 specifically recognizes and binds ligand-bound VDR when it is not associated with coactivators (NCOAs) (PubMed:37478846). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation (PubMed:37478846).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Terms & Conditions.
1 product image
Western blot - Recombinant Human Vitamin D Receptor protein (ab82068)
All lanes: Anti-Vitamin D Receptor antibody (ab82566) at 1 µg/mL
All lanes: Western blot - Recombinant Human Vitamin D Receptor protein (ab82068) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20s
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Product protocols
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