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Recombinant human Vitronectin/S-Protein (Animal Free) is a Human Full Length protein, in the 20 to 478 aa range, expressed in HEK 293, with >95% purity and suitable for SDS-PAGE, FuncS, HPLC.

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Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, HPLC

Biologically active

Yes

Amino acid sequence

D Q E S C K G R C T E G F N V D K K C Q C D E L C S Y Y Q S C C T D Y T A E C K P Q V T R G D V F T M P E D E Y T V Y D D G E E K N N A T V H E Q V G G P S L T S D L Q A Q S K G N P E Q T P V L K P E E E A P A P E V G A S K P E G I D S R P E T L H P G R P Q P P A E E E L C S G K P F D A F T D L K N G S L F A F R G Q Y C Y E L D E K A V R P G Y P K L I R D V W G I E G P I D A A F T R I N C Q G K T Y L F K G S Q Y W R F E D G V L D P D Y P R N I S D G F D G I P D N V D A A L A L P A H S Y S G R E R V Y F F K G K Q Y W E Y Q F Q H Q P S Q E E C E G S S L S A V F E H F A M M Q R D S W E D I F E L L F W G R T S A G T R Q P Q F I S R D W H G V P G Q V D A A M A G R I Y I S G M A P R P S L A K K Q R F R H R N R K G Y R S Q R G H S R G R N Q N S R R P S R A T W L S L F S S E E S N L G A N N Y D D Y R M D W L V P A T C E P I Q S V F F F S G D K Y Y R V N L R T R R V D T V D P P Y P R S I A Q Y W L G C P A P G H L

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

Alternative names

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Recombinant human Vitronectin/S-Protein (Animal Free) is a Human Full Length protein, in the 20 to 478 aa range, expressed in HEK 293, with >95% purity and suitable for SDS-PAGE, FuncS, HPLC.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, HPLC

Biological activity

Recombinant human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditionsat 5ug/ml.

Accession
P04004-1
Animal free

Yes

Species

Human

Reconstitution

Centrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Extended storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 5% Trehalose).

Concentration
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Sequence info

Amino acid sequence

D Q E S C K G R C T E G F N V D K K C Q C D E L C S Y Y Q S C C T D Y T A E C K P Q V T R G D V F T M P E D E Y T V Y D D G E E K N N A T V H E Q V G G P S L T S D L Q A Q S K G N P E Q T P V L K P E E E A P A P E V G A S K P E G I D S R P E T L H P G R P Q P P A E E E L C S G K P F D A F T D L K N G S L F A F R G Q Y C Y E L D E K A V R P G Y P K L I R D V W G I E G P I D A A F T R I N C Q G K T Y L F K G S Q Y W R F E D G V L D P D Y P R N I S D G F D G I P D N V D A A L A L P A H S Y S G R E R V Y F F K G K Q Y W E Y Q F Q H Q P S Q E E C E G S S L S A V F E H F A M M Q R D S W E D I F E L L F W G R T S A G T R Q P Q F I S R D W H G V P G Q V D A A M A G R I Y I S G M A P R P S L A K K Q R F R H R N R K G Y R S Q R G H S R G R N Q N S R R P S R A T W L S L F S S E E S N L G A N N Y D D Y R M D W L V P A T C E P I Q S V F F F S G D K Y Y R V N L R T R R V D T V D P P Y P R S I A Q Y W L G C P A P G H L

Accession

P04004

Protein length

Full Length

Predicted molecular weight

52.29 kDa

Amino acids

20 to 478

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>95% by HPLC.

General info

Function

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Post-translational modifications

Sulfated on tyrosine residues.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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