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AB131683

Recombinant Human VLK protein

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Recombinant Human VLK protein is a Human Fragment protein, in the 129 to 493 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB.

View Alternative Names

SGK493, VLK, PKDCC, Extracellular tyrosine-protein kinase PKDCC, Protein kinase-like protein SgK493, Sugen kinase 493, Vertebrate lonesome kinase

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SDS-PAGE - Recombinant Human VLK protein (AB131683)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human VLK protein (AB131683)

The purity of ab131683 was determined to be >70% by densitometry.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q504Y2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Protein kinase-like protein SgK493
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"69 kDa","actualMolecularWeight":null,"aminoAcidEnd":493,"aminoAcidStart":129,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q504Y2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VLK also known as V-set and transmembrane domain-containing protein E is a serine/threonine kinase with a molecular mass of approximately 54 kDa. It localizes mainly in the Golgi apparatus. Scientists detect VLK expression across various tissues including the heart testis and liver. This kinase executes post-translational modifications by phosphorylating intracellular proteins which can modulate their functions and interactions.
Biological function summary

VLK kinase activity impacts protein folding and trafficking within the Golgi apparatus. VLK associates with protein complexes involved in secretory pathways assisting cellular functions like protein secretion and cell surface expression. Research indicates that it contributes significantly to maintaining cellular homeostasis by ensuring proper protein maturation.

Pathways

VLK plays a role in the secretory pathway which is essential for the transport and delivery of proteins and lipids throughout the cell. It shows interactions with proteins like ABCD3 and VCP that are pivotal in vesicular transport and protein quality control. VLK also participates in the RAS signaling pathway linking it to cell growth and differentiation by affecting downstream targets.

Recent studies highlight VLK's involvement with neurodegenerative diseases and cancer. Altered VLK activity correlates with conditions such as Alzheimer's disease where phosphorylation imbalances disrupt neuron function. In cancer VLK is overexpressed leading to misregulated cell proliferation. Connections to proteins like tau and LATS1 further demonstrate VLK's relevance in these diseases influencing both progression and therapeutic targeting.
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Specifications

Form

Liquid

Additional notes

Assessed by densitometry. Affinity purified.

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General info

Function

Secreted tyrosine-protein kinase that mediates phosphorylation of extracellular proteins and endogenous proteins in the secretory pathway, which is essential for patterning at organogenesis stages. Mediates phosphorylation of MMP1, MMP13, MMP14, MMP19 and ERP29 (PubMed : 25171405). Probably plays a role in platelets : rapidly and quantitatively secreted from platelets in response to stimulation of platelet degranulation (PubMed : 25171405). May also have serine/threonine protein kinase activity. Required for longitudinal bone growth through regulation of chondrocyte differentiation. May be indirectly involved in protein transport from the Golgi apparatus to the plasma membrane (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily.

Post-translational modifications

N-glycosylated.. Phosphorylated on tyrosines; probably via autophosphorylation.

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Product protocols

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Target data

Secreted tyrosine-protein kinase that mediates phosphorylation of extracellular proteins and endogenous proteins in the secretory pathway, which is essential for patterning at organogenesis stages. Mediates phosphorylation of MMP1, MMP13, MMP14, MMP19 and ERP29 (PubMed : 25171405). Probably plays a role in platelets : rapidly and quantitatively secreted from platelets in response to stimulation of platelet degranulation (PubMed : 25171405). May also have serine/threonine protein kinase activity. Required for longitudinal bone growth through regulation of chondrocyte differentiation. May be indirectly involved in protein transport from the Golgi apparatus to the plasma membrane (By similarity).
See full target information PKDCC
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