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AB48702

Recombinant Human Von Hippel Lindau/VHL protein

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Recombinant Human Von Hippel Lindau/VHL protein is a Human Fragment protein, in the 1 to 154 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

von Hippel-Lindau disease tumor suppressor, Protein G7, pVHL, VHL

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SDS-PAGE - Recombinant Human Von Hippel Lindau/VHL protein (AB48702)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Von Hippel Lindau/VHL protein (AB48702)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P40337

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 0.0584% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLP","proteinLength":"Fragment","predictedMolecularWeight":"19.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":154,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P40337","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Von Hippel Lindau/VHL ß-domain (1-154) was overexpressed in E.coli and purified by using conventional chromatography techniques.

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General info

Function

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).

Sequence similarities

Belongs to the VHL family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 10944113, PubMed : 17981124, PubMed : 19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed : 10944113, PubMed : 17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed : 10944113, PubMed : 17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed : 19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed : 34290272).
See full target information VHL
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