Recombinant Human VP16 protein is a Human alphaherpesvirus 1 strain 17 Fragment protein, in the 411 to 490 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
S T A P P T D V S L G D E L H L D G E D V A M A H A D A L D D F D L D M L G D G D S P G P G F T P H D S A P Y G A L D M A D F E F E Q M F T D A L G I D E Y G G
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Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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In the early stage of viral replication, acts as a transcriptional activator of immediate-early (IE) gene products (alpha-genes), which is released by invading virions (PubMed:12826401, PubMed:23029222). Recruits P-TEFb to the viral alpha-gene promoters and overcomes transcriptional inhibition by ICP22 to promote transcription of IE genes (PubMed:15654739, PubMed:23029222). VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (PubMed:12826401). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F1 (PubMed:10398682). This complex recognizes the core motif 'TAATGARAT' in alpha-gene promoters (PubMed:12826401). In the late stage of viral replication, VP16, as a tegument, is involved in viral assembly (PubMed:19279114). May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.
VP16
Tegument protein VP16, Alpha trans-inducing protein, Alpha-TIF, ICP25, Vmw65, UL48
Recombinant Human VP16 protein is a Human alphaherpesvirus 1 strain 17 Fragment protein, in the 411 to 490 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA
Isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.
In the early stage of viral replication, acts as a transcriptional activator of immediate-early (IE) gene products (alpha-genes), which is released by invading virions (PubMed:12826401, PubMed:23029222). Recruits P-TEFb to the viral alpha-gene promoters and overcomes transcriptional inhibition by ICP22 to promote transcription of IE genes (PubMed:15654739, PubMed:23029222). VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (PubMed:12826401). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F1 (PubMed:10398682). This complex recognizes the core motif 'TAATGARAT' in alpha-gene promoters (PubMed:12826401). In the late stage of viral replication, VP16, as a tegument, is involved in viral assembly (PubMed:19279114).
Belongs to the herpesviridae tegument protein VP16 protein family.
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