Recombinant Human VPS25 protein is a Human Full Length protein, in the 1 to 176 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M A M S F E W P W Q Y R F P P F F T L Q P N V D T R Q K Q L A A W C S L V L S F C R L H K Q S S M T V M E A Q E S P L F N N V K L Q R K L P V E S I Q I V L E E L R K K G N L E W L D K S K S S F L I M W R R P E E W G K L I Y Q W V S R S G Q N N S V F T L Y E L T N G E D T E D E E F H G L D E A T L L R A L Q A L Q Q E H K A E I I T V S D G R G V K F F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
Alternative names
DERP9, EAP20, VPS25, Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25
Recommended products
Recombinant Human VPS25 protein is a Human Full Length protein, in the 1 to 176 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9BRG1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M A M S F E W P W Q Y R F P P F F T L Q P N V D T R Q K Q L A A W C S L V L S F C R L H K Q S S M T V M E A Q E S P L F N N V K L Q R K L P V E S I Q I V L E E L R K K G N L E W L D K S K S S F L I M W R R P E E W G K L I Y Q W V S R S G Q N N S V F T L Y E L T N G E D T E D E E F H G L D E A T L L R A L Q A L Q Q E H K A E I I T V S D G R G V K F F
- Accession
- Q9BRG1
- Protein length
- Full Length
- Predicted molecular weight
- 23.3 kDa
- Amino acids
- 1 to 176
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124321 was purified using conventional chromatography techniques.
General info
- Function
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
- Sequence similarities
Belongs to the VPS25 family.
- Subcellular localisation
- Endosome membrane, Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human VPS25 protein (ab124321)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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