Recombinant Human VPS26 protein is a Human Full Length protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S F L G G F F G P I C E I D I V L N D G E T R K M A E M K T E D G K V E K H Y L F Y D G E S V S G K V N L A F K Q P G K R L E H Q G I R I E F V G Q I E L F N D K S N T H E F V N L V K E L A L P G E L T Q S R S Y D F E F M Q V E K P Y E S Y I G A N V R L R Y F L K V T I V R R L T D L V K E Y D L I V H Q L A T Y P D V N N S I K M E V G I E D C L H I E F E Y N K S K Y H L K D V I V G K I Y F L L V R I K I Q H M E L Q L I K K E I T G I G P S T T T E T E T I A K Y E I M D G A P V K G E S I P I R L F L A G Y D P T P T M R D V N K K F S V R Y F L N L V L V D E E D R R Y F K Q Q E I I L W R K A P E K L R K Q R T N F H Q R F E S P E S Q A S A E Q P E M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins (Probable). The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R (PubMed:15078902, PubMed:15078903). Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15247922). Required for the endosomal localization of WASHC2A (indicative for the WASH complex) (PubMed:22070227). Required for the endosomal localization of TBC1D5 (PubMed:20923837). Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (PubMed:22279231). Involved in retromer-independent lysosomal sorting of F2R (PubMed:16407403). Involved in recycling of ADRB2 (PubMed:21602791). Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).
Alternative names
VPS26, VPS26A, Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26
Recommended products
Recombinant Human VPS26 protein is a Human Full Length protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O75436-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S F L G G F F G P I C E I D I V L N D G E T R K M A E M K T E D G K V E K H Y L F Y D G E S V S G K V N L A F K Q P G K R L E H Q G I R I E F V G Q I E L F N D K S N T H E F V N L V K E L A L P G E L T Q S R S Y D F E F M Q V E K P Y E S Y I G A N V R L R Y F L K V T I V R R L T D L V K E Y D L I V H Q L A T Y P D V N N S I K M E V G I E D C L H I E F E Y N K S K Y H L K D V I V G K I Y F L L V R I K I Q H M E L Q L I K K E I T G I G P S T T T E T E T I A K Y E I M D G A P V K G E S I P I R L F L A G Y D P T P T M R D V N K K F S V R Y F L N L V L V D E E D R R Y F K Q Q E I I L W R K A P E K L R K Q R T N F H Q R F E S P E S Q A S A E Q P E M
- Accession
- O75436
- Protein length
- Full Length
- Predicted molecular weight
- 40.6 kDa
- Amino acids
- 1 to 327
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab130047 was purified using conventional chromatography techniques.
General info
- Function
Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins (Probable). The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R (PubMed:15078902, PubMed:15078903). Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15247922). Required for the endosomal localization of WASHC2A (indicative for the WASH complex) (PubMed:22070227). Required for the endosomal localization of TBC1D5 (PubMed:20923837). Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (PubMed:22279231). Involved in retromer-independent lysosomal sorting of F2R (PubMed:16407403). Involved in recycling of ADRB2 (PubMed:21602791). Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).
- Sequence similarities
Belongs to the VPS26 family.
- Subcellular localisation
- Endosome membrane, Early endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Human VPS26 protein (ab130047)
15% SDS-PAGE analysis of 3 μg of Human VPS26 protein (ab130047)
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