Recombinant Human VPS33B protein is a Human Full Length protein, in the 1 to 617 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A F P H R P D A P E L P D F S M L K R L A R D Q L I Y L L E Q L P G K K D L F I E A D L M S P L D R I A N V S I L K Q H E V D K L Y K V E N K P A L S S N E Q L C F L V R P R I K N M R Y I A S L V N A D K L A G R T R K Y K V I F S P Q K F Y A C E M V L E E E G I Y G D V S C D E W A F S L L P L D V D L L S M E L P E F F R D Y F L E G D Q R W I N T V A Q A L H L L S T L Y G P F P N C Y G I G R C A K M A Y E L W R N L E E E E D G E T K G R R P E I G H I F L L D R D V D F V T A L C S Q V V Y E G L V D D T F R I K C G S V D F G P E V T S S D K S L K V L L N A E D K V F N E I R N E H F S N V F G F L S Q K A R N L Q A Q Y D R R R G M D I K Q M K N F V S Q E L K G L K Q E H R L L S L H I G A C E S I M K K K T K Q D F Q E L I K T E H A L L E G F N I R E S T S Y I E E H I D R Q V S P I E S L R L M C L L S I T E N G L I P K D Y R S L K T Q Y L Q S Y G P E H L L T F S N L R R A G L L T E Q A P G D T L T A V E S K V S K L V T D K A A G K I T D A F S S L A K R S N F R A I S K K L N L I P R V D G E Y D L K V P R D M A Y V F S G A Y V P L S C R I I E Q V L E R R S W Q G L D E V V R L L N C S D F A F T D M T K E D K A S S E S L R L I L V V F L G G C T F S E I S A L R F L G R E K G Y R F I F L T T A V T N S A R L M E A M S E V K A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Target data
Function
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen (PubMed:28017832). Mediates phagolysosomal fusion in macrophages (PubMed:18474358). Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity).
Alternative names
Vacuolar protein sorting-associated protein 33B, hVPS33B, VPS33B
Recommended products
Recombinant Human VPS33B protein is a Human Full Length protein, in the 1 to 617 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9H267-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A F P H R P D A P E L P D F S M L K R L A R D Q L I Y L L E Q L P G K K D L F I E A D L M S P L D R I A N V S I L K Q H E V D K L Y K V E N K P A L S S N E Q L C F L V R P R I K N M R Y I A S L V N A D K L A G R T R K Y K V I F S P Q K F Y A C E M V L E E E G I Y G D V S C D E W A F S L L P L D V D L L S M E L P E F F R D Y F L E G D Q R W I N T V A Q A L H L L S T L Y G P F P N C Y G I G R C A K M A Y E L W R N L E E E E D G E T K G R R P E I G H I F L L D R D V D F V T A L C S Q V V Y E G L V D D T F R I K C G S V D F G P E V T S S D K S L K V L L N A E D K V F N E I R N E H F S N V F G F L S Q K A R N L Q A Q Y D R R R G M D I K Q M K N F V S Q E L K G L K Q E H R L L S L H I G A C E S I M K K K T K Q D F Q E L I K T E H A L L E G F N I R E S T S Y I E E H I D R Q V S P I E S L R L M C L L S I T E N G L I P K D Y R S L K T Q Y L Q S Y G P E H L L T F S N L R R A G L L T E Q A P G D T L T A V E S K V S K L V T D K A A G K I T D A F S S L A K R S N F R A I S K K L N L I P R V D G E Y D L K V P R D M A Y V F S G A Y V P L S C R I I E Q V L E R R S W Q G L D E V V R L L N C S D F A F T D M T K E D K A S S E S L R L I L V V F L G G C T F S E I S A L R F L G R E K G Y R F I F L T T A V T N S A R L M E A M S E V K A
- Accession
- Q9H267
- Protein length
- Full Length
- Predicted molecular weight
- 97 kDa
- Amino acids
- 1 to 617
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen (PubMed:28017832). Mediates phagolysosomal fusion in macrophages (PubMed:18474358). Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity).
- Sequence similarities
Belongs to the STXBP/unc-18/SEC1 family.
- Post-translational modifications
Phosphorylated on tyrosine residues.
- Subcellular localisation
- Late endosome membrane, Lysosome membrane, Early endosome, Recycling endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human VPS33B protein (ab132028)
12.5% SDS-PAGE stained with Coomassie Blue showing ab132028 at approximately 97 kDa.
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