Recombinant human VPS34 protein is a Human Full Length protein, in the 2 to 887 aa range, expressed in Baculovirus infected Sf9, with >=70% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
G E A E K F H Y I Y S C D L D I N V Q L K I G S L E G K R E Q K S Y K A V L E D P M L K F S G L Y Q E T C S D L Y V T C Q V F A E G K P L A L P V R T S Y K A F S T R W N W N E W L K L P V K Y P D L P R N A Q V A L T I W D V Y G P G K A V P V G G T T V S L F G K Y G M F R Q G M H D L K V W P N V E A D G S E P T K T P G R T S S T L S E D Q M S R L A K L T K A H R Q G H M V K V D W L D R L T F R E I E M I N E S E K R S S N F M Y L M V E F R C V K C D D K E Y G I V Y Y E K D G D E S S P I L T S F E L V K V P D P Q M S M E N L V E S K H H K L A R S L R S G P S D H D L K P N A A T R D Q L N I I V S Y P P T K Q L T Y E E Q D L V W K F R Y Y L T N Q E K A L T K F L K C V N W D L P Q E A K Q A L E L L G K W K P M D V E D S L E L L S S H Y T N P T V R R Y A V A R L R Q A D D E D L L M Y L L Q L V Q A L K Y E N F D D I K N G L E P T K K D S Q S S V S E N V S N S G I N S A E I D S S Q I I T S P L P S V S S P P P A S K T K E V P D G E N L E Q D L C T F L I S R A C K N S T L A N Y L Y W Y V I V E C E D Q D T Q Q R D P K T H E M Y L N V M R R F S Q A L L K G D K S V R V M R S L L A A Q Q T F V D R L V H L M K A V Q R E S G N R K K K N E R L Q A L L G D N E K M N L S D V E L I P L P L E P Q V K I R G I I P E T A T L F K S A L M P A Q L F F K T E D G G K Y P V I F K H G D D L R Q D Q L I L Q I I S L M D K L L R K E N L D L K L T P Y K V L A T S T K H G F M Q F I Q S V P V A E V L D T E G S I Q N F F R K Y A P S E N G P N G I S A E V M D T Y V K S C A G Y C V I T Y I L G V G D R H L D N L L L T K T G K L F H I D F G Y I L G R D P K P L P P P M K L N K E M V E G M G G T Q S E Q Y Q E F R K Q C Y T A F L H L R R Y S N L I L N L F S L M V D A N I P D I A L E P D K T V K K V Q D K F R L D L S D E E A V H Y M Q S L I D E S V H A L F A A V V E Q I H K F A Q Y W R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:14617358, PubMed:33637724, PubMed:7628435). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed:32690950). Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity). (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Alternative names
VPS34, PIK3C3, Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34
Recommended products
Recombinant human VPS34 protein is a Human Full Length protein, in the 2 to 887 aa range, expressed in Baculovirus infected Sf9, with >=70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 140 pmol/min/μg.
- Accession
- Q8NEB9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- G E A E K F H Y I Y S C D L D I N V Q L K I G S L E G K R E Q K S Y K A V L E D P M L K F S G L Y Q E T C S D L Y V T C Q V F A E G K P L A L P V R T S Y K A F S T R W N W N E W L K L P V K Y P D L P R N A Q V A L T I W D V Y G P G K A V P V G G T T V S L F G K Y G M F R Q G M H D L K V W P N V E A D G S E P T K T P G R T S S T L S E D Q M S R L A K L T K A H R Q G H M V K V D W L D R L T F R E I E M I N E S E K R S S N F M Y L M V E F R C V K C D D K E Y G I V Y Y E K D G D E S S P I L T S F E L V K V P D P Q M S M E N L V E S K H H K L A R S L R S G P S D H D L K P N A A T R D Q L N I I V S Y P P T K Q L T Y E E Q D L V W K F R Y Y L T N Q E K A L T K F L K C V N W D L P Q E A K Q A L E L L G K W K P M D V E D S L E L L S S H Y T N P T V R R Y A V A R L R Q A D D E D L L M Y L L Q L V Q A L K Y E N F D D I K N G L E P T K K D S Q S S V S E N V S N S G I N S A E I D S S Q I I T S P L P S V S S P P P A S K T K E V P D G E N L E Q D L C T F L I S R A C K N S T L A N Y L Y W Y V I V E C E D Q D T Q Q R D P K T H E M Y L N V M R R F S Q A L L K G D K S V R V M R S L L A A Q Q T F V D R L V H L M K A V Q R E S G N R K K K N E R L Q A L L G D N E K M N L S D V E L I P L P L E P Q V K I R G I I P E T A T L F K S A L M P A Q L F F K T E D G G K Y P V I F K H G D D L R Q D Q L I L Q I I S L M D K L L R K E N L D L K L T P Y K V L A T S T K H G F M Q F I Q S V P V A E V L D T E G S I Q N F F R K Y A P S E N G P N G I S A E V M D T Y V K S C A G Y C V I T Y I L G V G D R H L D N L L L T K T G K L F H I D F G Y I L G R D P K P L P P P M K L N K E M V E G M G G T Q S E Q Y Q E F R K Q C Y T A F L H L R R Y S N L I L N L F S L M V D A N I P D I A L E P D K T V K K V Q D K F R L D L S D E E A V H Y M Q S L I D E S V H A L F A A V V E Q I H K F A Q Y W R K
- Accession
- Q8NEB9
- Protein length
- Full Length
- Predicted molecular weight
- 98.2 kDa
- Amino acids
- 2 to 887
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:14617358, PubMed:33637724, PubMed:7628435). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed:32690950). Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity).
- Sequence similarities
Belongs to the PI3/PI4-kinase family.
- Post-translational modifications
Ubiquitinated via 'Lys-29'- and 'Lys-48'-linked ubiquitination by UBE3C, promoting its degradation (PubMed:33637724). Deubiquitination by ZRANB1/TRABID promotes its stabilization, leading to autophagosome maturation (PubMed:33637724).
- Subcellular localisation
- Late endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
This product was previously labelled as PI 3 Kinase Class 3
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2 product images
SDS-PAGE - Recombinant human VPS34 protein (ab198071)
10% SDS-PAGE analysis of ab198071 (4 μg) with Coomassie staining.
Functional Studies - Recombinant human VPS34 protein (ab198071)
Example of specific activity of ab198071.
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