Recombinant Human VPS35 protein is a Human Full Length protein, in the 1 to 796 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P T T Q Q S P Q D E Q E K L L D E A I Q A V K V Q S F Q M K R C L D K N K L M D A L K H A S N M L G E L R T S M L S P K S Y Y E L Y M A I S D E L H Y L E V Y L T D E F A K G R K V A D L Y E L V Q Y A G N I I P R L Y L L I T V G V V Y V K S F P Q S R K D I L K D L V E M C R G V Q H P L R G L F L R N Y L L Q C T R N I L P D E G E P T D E E T T G D I S D S M D F V L L N F A E M N K L W V R M Q H Q G H S R D R E K R E R E R Q E L R I L V G T N L V R L S Q L E G V N V E R Y K Q I V L T G I L E Q V V N C R D A L A Q E Y L M E C I I Q V F P D E F H L Q T L N P F L R A C A E L H Q N V N V K N I I I A L I D R L A L F A H R E D G P G I P A D I K L F D I F S Q Q V A T V I Q S R Q D M P S E D V V S L Q V S L I N L A M K C Y P D R V D Y V D K V L E T T V E I F N K L N L E H I A T S S A V S K E L T R L L K I P V D T Y N N I L T V L K L K H F H P L F E Y F D Y E S R K S M S C Y V L S N V L D Y N T E I V S Q D Q V D S I M N L V S T L I Q D Q P D Q P V E D P D P E D F A D E Q S L V G R F I H L L R S E D P D Q Q Y L I L N T A R K H F G A G G N Q R I R F T L P P L V F A A Y Q L A F R Y K E N S K V D D K W E K K C Q K I F S F A H Q T I S A L I K A E L A E L P L R L F L Q G A L A A G E I G F E N H E T V A Y E F M S Q A F S L Y E D E I S D S K A Q L A A I T L I I G T F E R M K C F S E E N H E P L R T Q C A L A A S K L L K K P D Q G R A V S T C A H L F W S G R N T D K N G E E L H G G K R V M E C L K K A L K I A N Q C M D P S L Q V Q L F I E I L N R Y I Y F Y E K E N D A V T I Q V L N Q L I Q K I R E D L P N L E S S E E T E Q I N K H F H N T L E H L R L R R E S P E S E G P I Y E G L I L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (PubMed:30213940). The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of WASHC2C (PubMed:22070227, PubMed:28892079). Mediates the association of the CSC with the WASH complex via WASHC2 (PubMed:22070227, PubMed:24819384, PubMed:24980502). Required for the endosomal localization of TBC1D5 (PubMed:20923837). (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.
Alternative names
MEM3, TCCCTA00141, VPS35, Vacuolar protein sorting-associated protein 35, hVPS35, Maternal-embryonic 3, Vesicle protein sorting 35
Recommended products
Recombinant Human VPS35 protein is a Human Full Length protein, in the 1 to 796 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q96QK1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P T T Q Q S P Q D E Q E K L L D E A I Q A V K V Q S F Q M K R C L D K N K L M D A L K H A S N M L G E L R T S M L S P K S Y Y E L Y M A I S D E L H Y L E V Y L T D E F A K G R K V A D L Y E L V Q Y A G N I I P R L Y L L I T V G V V Y V K S F P Q S R K D I L K D L V E M C R G V Q H P L R G L F L R N Y L L Q C T R N I L P D E G E P T D E E T T G D I S D S M D F V L L N F A E M N K L W V R M Q H Q G H S R D R E K R E R E R Q E L R I L V G T N L V R L S Q L E G V N V E R Y K Q I V L T G I L E Q V V N C R D A L A Q E Y L M E C I I Q V F P D E F H L Q T L N P F L R A C A E L H Q N V N V K N I I I A L I D R L A L F A H R E D G P G I P A D I K L F D I F S Q Q V A T V I Q S R Q D M P S E D V V S L Q V S L I N L A M K C Y P D R V D Y V D K V L E T T V E I F N K L N L E H I A T S S A V S K E L T R L L K I P V D T Y N N I L T V L K L K H F H P L F E Y F D Y E S R K S M S C Y V L S N V L D Y N T E I V S Q D Q V D S I M N L V S T L I Q D Q P D Q P V E D P D P E D F A D E Q S L V G R F I H L L R S E D P D Q Q Y L I L N T A R K H F G A G G N Q R I R F T L P P L V F A A Y Q L A F R Y K E N S K V D D K W E K K C Q K I F S F A H Q T I S A L I K A E L A E L P L R L F L Q G A L A A G E I G F E N H E T V A Y E F M S Q A F S L Y E D E I S D S K A Q L A A I T L I I G T F E R M K C F S E E N H E P L R T Q C A L A A S K L L K K P D Q G R A V S T C A H L F W S G R N T D K N G E E L H G G K R V M E C L K K A L K I A N Q C M D P S L Q V Q L F I E I L N R Y I Y F Y E K E N D A V T I Q V L N Q L I Q K I R E D L P N L E S S E E T E Q I N K H F H N T L E H L R L R R E S P E S E G P I Y E G L I L
- Accession
- Q96QK1
- Protein length
- Full Length
- Predicted molecular weight
- 118.1 kDa
- Amino acids
- 1 to 796
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (PubMed:30213940). The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of WASHC2C (PubMed:22070227, PubMed:28892079). Mediates the association of the CSC with the WASH complex via WASHC2 (PubMed:22070227, PubMed:24819384, PubMed:24980502). Required for the endosomal localization of TBC1D5 (PubMed:20923837).
- Sequence similarities
Belongs to the VPS35 family.
- Subcellular localisation
- Endosome, Early endosome, Late endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human VPS35 protein (ab132953)
12.5% SDS-PAGE analysis of ab132953 stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com