Recombinant Human VPS4B/MIG1 protein is a Human Full Length protein, in the 1 to 444 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M S S T S P N L Q K A I D L A S K A A Q E D K A G N Y E E A L Q L Y Q H A V Q Y F L H V V K Y E A Q G D K A K Q S I R A K C T E Y L D R A E K L K E Y L K N K E K K A Q K P V K E G Q P S P A D E K G N D S D G E G E S D D P E K K K L Q N Q L Q G A I V I E R P N V K W S D V A G L E G A K E A L K E A V I L P I K F P H L F T G K R T P W R G I L L F G P P G T G K S Y L A K A V A T E A N N S T F F S I S S S D L V S K W L G E S E K L V K N L F Q L A R E N K P S I I F I D E I D S L C G S R S E N E S E A A R R I K T E F L V Q M Q G V G V D N D G I L V L G A T N I P W V L D S A I R R R F E K R I Y I P L P E P H A R A A M F K L H L G T T Q N S L T E A D F R E L G R K T D G Y S G A D I S I I V R D A L M Q P V R K V Q S A T H F K K V R G P S R A D P N H L V D D L L T P C S P G D P G A I E M T W M D V P G D K L L E P V V S M S D M L R S L S N T K P T V N E H D L L K L K K F T E D F G Q E G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed:18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
Alternative names
SKD1, VPS42, MIG1, VPS4B, Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1
Recommended products
Recombinant Human VPS4B/MIG1 protein is a Human Full Length protein, in the 1 to 444 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75351-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M S S T S P N L Q K A I D L A S K A A Q E D K A G N Y E E A L Q L Y Q H A V Q Y F L H V V K Y E A Q G D K A K Q S I R A K C T E Y L D R A E K L K E Y L K N K E K K A Q K P V K E G Q P S P A D E K G N D S D G E G E S D D P E K K K L Q N Q L Q G A I V I E R P N V K W S D V A G L E G A K E A L K E A V I L P I K F P H L F T G K R T P W R G I L L F G P P G T G K S Y L A K A V A T E A N N S T F F S I S S S D L V S K W L G E S E K L V K N L F Q L A R E N K P S I I F I D E I D S L C G S R S E N E S E A A R R I K T E F L V Q M Q G V G V D N D G I L V L G A T N I P W V L D S A I R R R F E K R I Y I P L P E P H A R A A M F K L H L G T T Q N S L T E A D F R E L G R K T D G Y S G A D I S I I V R D A L M Q P V R K V Q S A T H F K K V R G P S R A D P N H L V D D L L T P C S P G D P G A I E M T W M D V P G D K L L E P V V S M S D M L R S L S N T K P T V N E H D L L K L K K F T E D F G Q E G
- Accession
- O75351
- Protein length
- Full Length
- Predicted molecular weight
- 51.8 kDa
- Amino acids
- 1 to 444
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab171483 was purified using conventional chromatography techniques.
General info
- Function
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed:18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
- Sequence similarities
Belongs to the AAA ATPase family.
- Subcellular localisation
- Late endosome membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as VPS4B
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1 product image
SDS-PAGE - Recombinant Human VPS4B/MIG1 protein (ab171483)
SDS-PAGE analysis of Recombinant Human VPS4B protein (ab171483), under reducing conditions. Proteins visualized by coomassie blue stain.
Lane 1: Molecular Weight Standards
Lane 2: 3 μg Recombinant Human VPS4B protein
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