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AB125555

Recombinant human VRK1 protein

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(1 Publication)

Recombinant human VRK1 protein is a Human Full Length protein, in the 1 to 396 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1

2 Images
Functional Studies - Recombinant human VRK1 protein (AB125555)
  • FuncS

Unknown

Functional Studies - Recombinant human VRK1 protein (AB125555)

Sample Kinase Assay showing the Specific Activity of ab125555 to be 2 nmol/min/mg.

SDS-PAGE - Recombinant human VRK1 protein (AB125555)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human VRK1 protein (AB125555)

SDS-PAGE analysis of ab125555.

Key facts

Purity

>95% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

The specific activity of ab125555 was determined to be 2 nmol/min/mg.

Accession

Q99986

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"71 kDa","actualMolecularWeight":null,"aminoAcidEnd":396,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q99986","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >95% by densitometry.

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General info

Function

Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed : 14645249, PubMed : 18617507, PubMed : 19103756, PubMed : 33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed : 31527692, PubMed : 37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed : 33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed : 21920476, PubMed : 31090908, PubMed : 31527692). Involved in Golgi disassembly during the cell cycle : following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed : 19103756). Phosphorylates BANF1 : disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed : 16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed : 10951572, PubMed : 31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed : 15105425). Phosphorylates JUN (PubMed : 31527692).

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

Post-translational modifications

Autophosphorylated at various serine and threonine residues (PubMed:11883897, PubMed:14645249, PubMed:19103756, PubMed:21543316). Autophosphorylation does not impair its ability to phosphorylate p53/TP53 (PubMed:11883897). Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis (PubMed:19103756).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed : 14645249, PubMed : 18617507, PubMed : 19103756, PubMed : 33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed : 31527692, PubMed : 37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed : 33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed : 21920476, PubMed : 31090908, PubMed : 31527692). Involved in Golgi disassembly during the cell cycle : following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed : 19103756). Phosphorylates BANF1 : disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed : 16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed : 10951572, PubMed : 31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed : 15105425). Phosphorylates JUN (PubMed : 31527692).
See full target information VRK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:11210 PubMed35778595

2022

Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tyrell N Cartwright,Rebecca J Harris,Stephanie K Meyer,Aye M Mon,Nikolaus A Watson,Cheryl Tan,Agathe Marcelot,Fangwei Wang,Sophie Zinn-Justin,Paula Traktman,Jonathan M G Higgins
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