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AB107698

Recombinant human VRK2 protein

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Recombinant human VRK2 protein is a Human Fragment protein, in the 1 to 375 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Serine/threonine-protein kinase VRK2, Vaccinia-related kinase 2, VRK2

4 Images
Functional Studies - Recombinant human VRK2 protein (AB107698)
  • FuncS

Unknown

Functional Studies - Recombinant human VRK2 protein (AB107698)

Specific activity of ab107698 was determined to be 12 nmol/min/mg

Functional Studies - Recombinant human VRK2 protein (AB107698)
  • FuncS

Unknown

Functional Studies - Recombinant human VRK2 protein (AB107698)

The specific activity of VRK2 (ab107698) was determined to be 10 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human VRK2 protein (AB107698)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human VRK2 protein (AB107698)

SDS-PAGE analysis of ab107698

SDS-PAGE - Recombinant human VRK2 protein (AB107698)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human VRK2 protein (AB107698)

SDS PAGE analysis of ab107698

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity: 12 nmol/min/mg

Accession

Q86Y07

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab91092 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"66 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q86Y07","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

The purity was determined to be >90% by densitometry. Affinity purified.

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General info

Function

Serine/threonine kinase that regulates several signal transduction pathways (PubMed : 14645249, PubMed : 16495336, PubMed : 16704422, PubMed : 17709393, PubMed : 18286207, PubMed : 18617507, PubMed : 20679487). Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes (PubMed : 17709393). Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription (PubMed : 18286207). Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1 (PubMed : 16704422). Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins (PubMed : 16495336). Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1 (PubMed : 20679487). Blocks the phosphorylation of ERK in response to ERBB2 and HRAS (PubMed : 20679487). Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity : casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (PubMed : 14645249).. Isoform 2. Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

Post-translational modifications

Isoform 1. Autophosphorylated.. Isoform 2. Autophosphorylated.

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Product protocols

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Target data

Serine/threonine kinase that regulates several signal transduction pathways (PubMed : 14645249, PubMed : 16495336, PubMed : 16704422, PubMed : 17709393, PubMed : 18286207, PubMed : 18617507, PubMed : 20679487). Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes (PubMed : 17709393). Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription (PubMed : 18286207). Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1 (PubMed : 16704422). Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins (PubMed : 16495336). Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1 (PubMed : 20679487). Blocks the phosphorylation of ERK in response to ERBB2 and HRAS (PubMed : 20679487). Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity : casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (PubMed : 14645249).. Isoform 2. Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.
See full target information Serine/threonine-protein kinase VRK2
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