Recombinant human VRK2 protein is a Human Fragment protein, in the 1 to 375 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.Isoform 2 phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.
Alternative names
Serine/threonine-protein kinase VRK2, Vaccinia-related kinase 2, VRK2
Recommended products
Recombinant human VRK2 protein is a Human Fragment protein, in the 1 to 375 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Specific activity: 12 nmol/min/mg
- Accession
- Q86Y07-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- Protein length
Fragment
- Predicted molecular weight
66 kDa
- Amino acids
1 to 375
- Nature
Recombinant
Specifications
- Form
Liquid
- Additional notes
The purity was determined to be >90% by densitometry. Affinity purified.
General info
- Function
Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.
- Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
- Post-translational modifications
Isoform 1 and isoform 2 are autophosphorylated.
- Subcellular localisation
Mitochondrion membrane, Nucleus envelope, Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Cow Casein protein ab91092 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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4 product images
Functional Studies - Recombinant human VRK2 protein (ab107698)
The specific activity of VRK2 (ab107698) was determined to be 10 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human VRK2 protein (ab107698)
SDS PAGE analysis of ab107698
SDS-PAGE - Recombinant human VRK2 protein (ab107698)
SDS-PAGE analysis of ab107698
Functional Studies - Recombinant human VRK2 protein (ab107698)
Specific activity of ab107698 was determined to be 12 nmol/min/mg
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