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AB98079

Recombinant Human WDR5 protein (His tag N-Terminus)

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(2 Publications)

Recombinant Human WDR5 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

BIG3, WDR5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

1 Images
SDS-PAGE - Recombinant Human WDR5 protein (His tag N-Terminus) (AB98079)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human WDR5 protein (His tag N-Terminus) (AB98079)

15% SDS-PAGE analysis of 3µg ab98079

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P61964

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Primary Antibodies

AB178410

Anti-WDR5 antibody [EPR11350] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

  • 0
  • 0
Primary Antibodies

AB137033

Anti-ERCC8 antibody [EPR9237]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (AB137033)

  • 0
  • 0
Proteins & Peptides

AB269117

Recombinant human USP16 protein (Active)

Functional Studies - Recombinant human USP16 protein (Active) (AB269117)

  • 0
  • 0
Proteins & Peptides

AB236192

Recombinant Human SMARCA2 / BRM protein (Tagged)

SDS-PAGE - Recombinant Human SMARCA2 / BRM protein (Tagged) (AB236192)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC","proteinLength":"Full Length","predictedMolecularWeight":"38.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":334,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61964","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.
Biological function summary

WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.

Pathways

WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.

WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.
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Specifications

Form

Liquid

Additional notes

ab98079 was purified using conventional chromatography techniques.

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General info

Function

Contributes to histone modification (PubMed : 16600877, PubMed : 16829960, PubMed : 19103755, PubMed : 19131338, PubMed : 19556245, PubMed : 20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed : 16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 19103755, PubMed : 20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

Sequence similarities

Belongs to the WD repeat WDR5/wds family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Contributes to histone modification (PubMed : 16600877, PubMed : 16829960, PubMed : 19103755, PubMed : 19131338, PubMed : 19556245, PubMed : 20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed : 16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 19103755, PubMed : 20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).
See full target information WDR5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 39:110836 PubMed35584667

2022

H3-K27M-mutant nucleosomes interact with MLL1 to shape the glioma epigenetic landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Noa Furth,Danielle Algranati,Bareket Dassa,Olga Beresh,Vadim Fedyuk,Natasha Morris,Lawryn H Kasper,Dan Jones,Michelle Monje,Suzanne J Baker,Efrat Shema

mBio 12:e0343121 PubMed34933446

2021

GRWD1-WDR5-MLL2 Epigenetic Complex Mediates H3K4me3 Mark and Is Essential for Kaposi's Sarcoma-Associated Herpesvirus-Induced Cellular Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wei,Songjian Lu,Lifan Liang,Xian Wang,Wan Li,Tingting Li,Luping Chen,Enguo Ju,Xinquan Zhang,Zhao Lai,Yufei Huang,Xinghua Lu,Shou-Jiang Gao
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Product promise

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