Recombinant Human WDR5 protein is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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- Cell reports 39:1108362022H3-K27M-mutant nucleosomes interact with MLL1 to shape the glioma epigenetic landscape.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoa Furth,Danielle Algranati,Bareket Dassa,Olga Beresh,Vadim Fedyuk,Natasha Morris,Lawryn H Kasper,Dan Jones,Michelle Monje,Suzanne J Baker,Efrat ShemaPubMed 35584667
- mBio 12:e03431212021GRWD1-WDR5-MLL2 Epigenetic Complex Mediates H3K4me3 Mark and Is Essential for Kaposi's Sarcoma-Associated Herpesvirus-Induced Cellular Transformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Wei,Songjian Lu,Lifan Liang,Xian Wang,Wan Li,Tingting Li,Luping Chen,Enguo Ju,Xinquan Zhang,Zhao Lai,Yufei Huang,Xinghua Lu,Shou-Jiang GaoPubMed 34933446
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A T E E K K P E T E A A R A Q P T P S S S A T Q S K P T P V K P N Y A L K F T L A G H T K A V S S V K F S P N G E W L A S S S A D K L I K I W G A Y D G K F E K T I S G H K L G I S D V A W S S D S N L L V S A S D D K T L K I W D V S S G K C L K T L K G H S N Y V F C C N F N P Q S N L I V S G S F D E S V R I W D V K T G K C L K T L P A H S D P V S A V H F N R D G S L I V S S S Y D G L C R I W D T A S G Q C L K T L I D D D N P P V S F V K F S P N G K Y I L A A T L D N T L K L W D Y S K G K C L K T Y T G H K N E K Y C I F A N F S V T G G K W I V S G S E D N L V Y I W N L Q T K E I V Q K L Q G H T D V V I S T A C H P T E N I I A S A A L E N D K T I K L W K S D C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Contributes to histone modification (PubMed:16600877, PubMed:16829960, PubMed:19103755, PubMed:19131338, PubMed:19556245, PubMed:20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed:16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:19103755, PubMed:20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
Alternative names
BIG3, WDR5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
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Recombinant Human WDR5 protein is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P61964-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A T E E K K P E T E A A R A Q P T P S S S A T Q S K P T P V K P N Y A L K F T L A G H T K A V S S V K F S P N G E W L A S S S A D K L I K I W G A Y D G K F E K T I S G H K L G I S D V A W S S D S N L L V S A S D D K T L K I W D V S S G K C L K T L K G H S N Y V F C C N F N P Q S N L I V S G S F D E S V R I W D V K T G K C L K T L P A H S D P V S A V H F N R D G S L I V S S S Y D G L C R I W D T A S G Q C L K T L I D D D N P P V S F V K F S P N G K Y I L A A T L D N T L K L W D Y S K G K C L K T Y T G H K N E K Y C I F A N F S V T G G K W I V S G S E D N L V Y I W N L Q T K E I V Q K L Q G H T D V V I S T A C H P T E N I I A S A A L E N D K T I K L W K S D C
- Accession
- P61964
- Protein length
- Full Length
- Predicted molecular weight
- 38.8 kDa
- Amino acids
- 1 to 334
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab98079 was purified using conventional chromatography techniques.
General info
- Function
Contributes to histone modification (PubMed:16600877, PubMed:16829960, PubMed:19103755, PubMed:19131338, PubMed:19556245, PubMed:20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed:16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:19103755, PubMed:20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
- Sequence similarities
Belongs to the WD repeat WDR5/wds family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.
- Biological function summary
WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.
- Pathways
WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.
- Associated diseases and disorders
WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.
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SDS-PAGE - Recombinant Human WDR5 protein (ab98079)
15% SDS-PAGE analysis of 3µg ab98079
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