Recombinant human Wee1 protein (Tagged) is a Human Fragment protein, in the 215 to 646 aa range, expressed in Baculovirus infected Sf9, with >=75% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>=75% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
D T E K S G K R E F D V R Q T P Q V N I N P F T P D S L L L H S S G Q C R R R K R T Y W N D S C G E D M E A S D Y E L E D E T R P A K R I T I T E S N M K S R Y T T E F H E L E K I G S G E F G S V F K C V K R L D G C I Y A I K R S K K P L A G S V D E Q N A L R E V Y A H A V L G Q H S H V V R Y F S A W A E D D H M L I Q N E Y C N G G S L A D A I S E N Y R I M S Y F K E A E L K D L L L Q V G R G L R Y I H S M S L V H M D I K P S N I F I S R T S I P N A A S E E G D E D D W A S N K V M F K I G D L G H V T R I S S P Q V E E G D S R F L A N E V L Q E N Y T H L P K A D I F A L A L T V V C A A G A E P L P R N G D Q W H E I R Q G R L P R I P Q V L S Q E F T E L L K V M I H P D P E R R P S A M A L V K H S V L L S A S R K S A E Q L R I E L N A E K F K N S L L Q K E L K K A Q M A K A A A E E R A L F T D R M A T R S T T Q S N R T S R L I G K K M N R S V S L T I Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Alternative names
Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Recommended products
Recombinant human Wee1 protein (Tagged) is a Human Fragment protein, in the 215 to 646 aa range, expressed in Baculovirus infected Sf9, with >=75% purity and suitable for SDS-PAGE, FuncS.
Alternative names
Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Key facts
- Purity
>=75% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Specific Activity: ≥42 pmol/min/μg
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Lys4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.
- Accession
- P30291-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.06% Glutathione, 0.04% Tween, 0.02% Potassium chloride
Sequence info
Amino acid sequence
D T E K S G K R E F D V R Q T P Q V N I N P F T P D S L L L H S S G Q C R R R K R T Y W N D S C G E D M E A S D Y E L E D E T R P A K R I T I T E S N M K S R Y T T E F H E L E K I G S G E F G S V F K C V K R L D G C I Y A I K R S K K P L A G S V D E Q N A L R E V Y A H A V L G Q H S H V V R Y F S A W A E D D H M L I Q N E Y C N G G S L A D A I S E N Y R I M S Y F K E A E L K D L L L Q V G R G L R Y I H S M S L V H M D I K P S N I F I S R T S I P N A A S E E G D E D D W A S N K V M F K I G D L G H V T R I S S P Q V E E G D S R F L A N E V L Q E N Y T H L P K A D I F A L A L T V V C A A G A E P L P R N G D Q W H E I R Q G R L P R I P Q V L S Q E F T E L L K V M I H P D P E R R P S A M A L V K H S V L L S A S R K S A E Q L R I E L N A E K F K N S L L Q K E L K K A Q M A K A A A E E R A L F T D R M A T R S T T Q S N R T S R L I G K K M N R S V S L T I Y
- Accession
- Protein length
Fragment
- Predicted molecular weight
75 kDa
- Amino acids
215 to 646
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
- Post-translational modifications
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wee1 also known as Wee1-like protein kinase or WEE1 serves as an important regulator of cell cycle progression. Wee1 is a protein kinase with a molecular mass of approximately 96 kDa involved in the regulation of the cell cycle by inhibiting the entry into mitosis through phosphorylation of cyclin-dependent kinase 1 (CDK1). Expression of Wee1 occurs throughout various tissues but it is especially important in those that require tight control over cell division like the brain and reproductive organs. By suppressing premature mitosis Wee1 ensures cells have adequate time for DNA repair and completion of critical processes before cell division.
- Biological function summary
The function of Wee1 extends to its role in maintaining genomic stability. Wee1 operates as part of a regulatory complex and its inhibition results in defective cell cycle arrest potentially leading to DNA damage. The kinase acts to prevent transitions from the G2 to M phase of the cell cycle ensuring cells repair damaged DNA before division. In the context of DNA replication stress Wee1 cooperates with other regulators such as Chk1 to mediate cell cycle arrest therefore safeguarding genomic integrity.
- Pathways
The role of Wee1 manifests significantly within the DNA damage checkpoint pathway and the cell cycle control pathway. In the DNA damage checkpoint pathway Wee1 collaborates with other cell cycle regulators such as ATR and Chk1 to control the cell cycle in response to DNA damages. Wee1's influence on the cell cycle pathway also intersects with CDK1 and Cyclin B where Wee1 modulates the activity of these proteins to control cell cycle transitions. This regulatory action allows cells to coordinate DNA repair and replication with cell division events.
- Associated diseases and disorders
Wee1's regulatory functions relate closely to cancer and neurological disorders. Overexpression or mutation of Wee1 is associated with various cancers including gliomas and breast cancer where it influences cell proliferation by controlling the activity of CDK1. Wee1's relationship with cancer extends to its interactions with p53 and Chk1 proteins both of which are critical in cancer biology. Additionally anomalies in Wee1 expression or function also associate with certain neurological disorders where it may alter cell cycle dynamics and influence neural cell fate under stress conditions.
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2 product images
SDS-PAGE - Recombinant human Wee1 protein (Tagged) (ab271795)
SDS-PAGE analysis of ab271795.
Functional Studies - Recombinant human Wee1 protein (Tagged) (ab271795)
Specific activity of ab271795 was ≥42 pmol/min/μg.
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