Recombinant Human WHSC1/NSD2 protein is a Human Full Length protein, in the 1 to 647 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M E F S I K Q S P L S V Q S V V K C I K M K Q A P E I L G S A N G K T P S C E V N R E C S V F L S K A Q L S S S L Q E G V M Q K F N G H D A L P F I P A D K L K D L T S R V F N G E P G A H D A K L R F E S Q E M K G I G T P P N T T P I K N G S P E I K L K I T K T Y M N G K P L F E S S I C G D S A A D V S Q S E E N G Q K P E N K A R R N R K R S I K Y D S L L E Q G L V E A A L V S K I S S P S D K K I P A K K E S C P N T G R D K D H L L K Y N V G D L V W S K V S G Y P W W P C M V S A D P L L H S Y T K L K G Q K K S A R Q Y H V Q F F G D A P E R A W I F E K S L V A F E G E G Q F E K L C Q E S A K Q A P T K A E K I K L L K P I S G K L R A Q W E M G I V Q A E E A A S M S V E E R K A K F T F L Y V G D Q L H L N P Q V A K E A G I A A E S L G E M A E S S G V S E E A A E N P K S V R E E C I P M K R R R R A K L C S S A E T L E S H P D I G K S T P Q K T A E A D P R R G V G S P P G R K K T T V S M P R S R K G D A A S Q F L V F C Q K H R D E V V A E H P D A S G E E I E E L L R S Q W S L L S E K Q R A R Y N T K F A L V A P V Q A E E D S G N V N G K K R N H T K R I Q D P T E D A E A E D T P R K R L R T D K H S L R K R D T I T D K T A R T S S Y K A M E A A S S L K S Q A A T K N L S D A C K P L K K R N R A S T A A S S A L G F S K S S S P S A S L T E N E L L W E P T P V K L D L N P A A L Y C T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed:19808676, PubMed:22099308, PubMed:27571355, PubMed:29728617, PubMed:33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed:22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed:22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed:16115125, PubMed:22099308, PubMed:29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed:29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity). Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2). Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed:22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed:18172012, PubMed:22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed:18172012). Does not methylate histone H3 at 'Lys-27' (PubMed:22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed:11152655, PubMed:18172012).
Alternative names
KIAA1090, MMSET, TRX5, WHSC1, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein
Recommended products
Recombinant Human WHSC1/NSD2 protein is a Human Full Length protein, in the 1 to 647 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- O96028-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E F S I K Q S P L S V Q S V V K C I K M K Q A P E I L G S A N G K T P S C E V N R E C S V F L S K A Q L S S S L Q E G V M Q K F N G H D A L P F I P A D K L K D L T S R V F N G E P G A H D A K L R F E S Q E M K G I G T P P N T T P I K N G S P E I K L K I T K T Y M N G K P L F E S S I C G D S A A D V S Q S E E N G Q K P E N K A R R N R K R S I K Y D S L L E Q G L V E A A L V S K I S S P S D K K I P A K K E S C P N T G R D K D H L L K Y N V G D L V W S K V S G Y P W W P C M V S A D P L L H S Y T K L K G Q K K S A R Q Y H V Q F F G D A P E R A W I F E K S L V A F E G E G Q F E K L C Q E S A K Q A P T K A E K I K L L K P I S G K L R A Q W E M G I V Q A E E A A S M S V E E R K A K F T F L Y V G D Q L H L N P Q V A K E A G I A A E S L G E M A E S S G V S E E A A E N P K S V R E E C I P M K R R R R A K L C S S A E T L E S H P D I G K S T P Q K T A E A D P R R G V G S P P G R K K T T V S M P R S R K G D A A S Q F L V F C Q K H R D E V V A E H P D A S G E E I E E L L R S Q W S L L S E K Q R A R Y N T K F A L V A P V Q A E E D S G N V N G K K R N H T K R I Q D P T E D A E A E D T P R K R L R T D K H S L R K R D T I T D K T A R T S S Y K A M E A A S S L K S Q A A T K N L S D A C K P L K K R N R A S T A A S S A L G F S K S S S P S A S L T E N E L L W E P T P V K L D L N P A A L Y C T
- Accession
- O96028
- Protein length
- Full Length
- Amino acids
- 1 to 647
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed:19808676, PubMed:22099308, PubMed:27571355, PubMed:29728617, PubMed:33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed:22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed:22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed:16115125, PubMed:22099308, PubMed:29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed:29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity).
- Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.
- Biological function summary
WHSC1/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1/NSD2 plays a significant role in development and cellular responses to environmental signals.
- Pathways
WHSC1/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.
- Associated diseases and disorders
Alterations in WHSC1/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.
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1 product image
SDS-PAGE - Recombinant Human WHSC1/NSD2 protein (ab159810)
ab159810 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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