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AB198120

Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus)

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Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus) is a Human Fragment protein, in the 941 to 1240 aa range, expressed in Escherichia coli, with >82%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA1090, MMSET, TRX5, WHSC1, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein

2 Images
Functional Studies - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus) (AB198120)
  • FuncS

Supplier Data

Functional Studies - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus) (AB198120)

Specific activity of ab198120 was determined to be 0.036 pmol/min/μg.

SDS-PAGE - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus) (AB198120)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus) (AB198120)

4-20% SDS-PAGE stained with Coomassie Blue.
Lane 1 : ab198120 (2 μg)
Lane 2 : Protein Marker

Key facts

Purity

>82% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.036 pmol/min/μg

Assay Conditions: Add 50 μL reaction mix (100 μM S-adenosylmethionine, and 50-250 ng WHSC1/NSD2 in HMT buffer 7) to wells coated with the substrate. Incubate at 37oC for 2 hours. Add antibody against methylated K36 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence.

Accession

O96028

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Primary Antibodies

AB75359

Anti-WHSC1/NSD2 antibody [29D1]

Trial Size

Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)

  • 5
  • 4
Proteins & Peptides

AB196419

Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus)

Functional Studies - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

  • 0
  • 0
Proteins & Peptides

AB198113

Recombinant Human KAT2A / GCN5 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human KAT2A / GCN5 protein (His tag N-Terminus) (AB198113)

  • 0
  • 0
Proteins & Peptides

AB162449

Recombinant Human CRBN protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CRBN protein (GST tag N-Terminus) (AB162449)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEGDRGSRYQGVRGIGRVFKNALQEAEARFREIKLQREARETQESERKPPPYKHIKVNKPYGKVQIYTADISEIPKCNCKPTDENPCGFDSECLNRMLMFECHPQVCPAGEFCQNQCFTKRQYPETKIIKTDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIKHAHENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGDRPKTSTTLSSEEKGKKTKKKTRRRRAKGEGKRQSED","proteinLength":"Fragment","predictedMolecularWeight":"62 kDa","actualMolecularWeight":null,"aminoAcidEnd":1240,"aminoAcidStart":941,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O96028","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.
Biological function summary

WHSC1/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1/NSD2 plays a significant role in development and cellular responses to environmental signals.

Pathways

WHSC1/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.

Alterations in WHSC1/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.
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Specifications

Form

Liquid

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General info

Function

Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 19808676, PubMed : 22099308, PubMed : 27571355, PubMed : 29728617, PubMed : 33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed : 22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed : 22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed : 16115125, PubMed : 22099308, PubMed : 29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed : 29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity).. Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2).. Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed : 18172012, PubMed : 22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed : 18172012). Does not methylate histone H3 at 'Lys-27' (PubMed : 22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed : 11152655, PubMed : 18172012).

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 19808676, PubMed : 22099308, PubMed : 27571355, PubMed : 29728617, PubMed : 33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed : 22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed : 22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed : 16115125, PubMed : 22099308, PubMed : 29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed : 29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity).. Isoform 1. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2).. Isoform 4. Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed : 22099308). Methylation of histone H3 at 'Lys-27' is controversial (PubMed : 18172012, PubMed : 22099308). Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3) (PubMed : 18172012). Does not methylate histone H3 at 'Lys-27' (PubMed : 22099308). May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment (PubMed : 11152655, PubMed : 18172012).
See full target information NSD2
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