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AB116197

Recombinant Human WHSC2/NELF-A protein

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Recombinant Human WHSC2/NELF-A protein is a Human Full Length protein, in the 1 to 539 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

WHSC2, P/OKcl.15, NELFA, Negative elongation factor A, NELF-A, Wolf-Hirschhorn syndrome candidate 2 protein

1 Images
SDS-PAGE - Recombinant Human WHSC2/NELF-A protein (AB116197)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human WHSC2/NELF-A protein (AB116197)

15% SDS-PAGE analysis of 3 μg ab116197.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9H3P2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein previously labeled as WHSC2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPGQRRALSPKMASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS","proteinLength":"Full Length","predictedMolecularWeight":"60.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":539,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H3P2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WHSC2 also known as NELF-A mechanically operates as a transcription elongation factor. It is a 66 kDa protein expressed in various tissues throughout the human body with higher levels in the brain and testis. WHSC2 is part of the negative elongation factor (NELF) complex. This complex interacts with RNA polymerase II (RNAPII) to pause transcription elongation an important step in regulating gene expression.
Biological function summary

WHSC2 plays a significant role in maintaining transcriptional pausing which affects gene expression. It is a component of the NELF complex which includes NELF-B NELF-C/D and NELF-E. By pausing RNAPII WHSC2 regulates the timing of transcription and impacts processes like differentiation and cell cycle control. This function is important for responding to environmental signals and cellular requirements.

Pathways

WHSC2 participates in the transcription regulation pathway and the cell cycle control pathway. It is related to proteins such as DSIF which also influences RNA polymerase II pausing. By being part of these pathways WHSC2 exerts control over the transition from transcription initiation to elongation impacting the expression timing of many genes. This positioning allows it to coordinate with other regulatory proteins and respond to cellular changes.

WHSC2 links to Wolf-Hirschhorn syndrome and various cancers. In Wolf-Hirschhorn syndrome deletions affecting WHSC2 contribute to developmental delays and growth retardation. In cancer altered WHSC2 expression is associated with abnormal cell growth particularly through interactions with p53 a tumor suppressor protein. The relationship highlights the importance of WHSC2 in both genetic disorders and oncogenic pathways.
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Specifications

Form

Liquid

Additional notes

ab116197 was purified using conventional chromatography techniques.

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General info

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

Sequence similarities

Belongs to the NELF-A family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
See full target information NELFA
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