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AB132512

Recombinant Human WIPF1 protein

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(2 Publications)

Recombinant Human WIPF1 protein is a Human Full Length protein, in the 1 to 358 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

WASPIP, WIP, WIPF1, WAS/WASL-interacting protein family member 1, Protein PRPL-2, Wiskott-Aldrich syndrome protein-interacting protein, WASP-interacting protein

1 Images
SDS-PAGE - Recombinant Human WIPF1 protein (AB132512)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human WIPF1 protein (AB132512)

12.5% SDS-PAGE analysis of ab132512 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

O43516

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNHSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHNRGSPPVPGGPRQPSPGPTPPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPPIPR","proteinLength":"Full Length","predictedMolecularWeight":"62.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":358,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O43516","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The WIPF1 protein also known as WASP Interacting Protein or WIP plays a mechanical role in the regulation and stabilization of the actin cytoskeleton. It weighs approximately 65 kDa. Scientists find WIPF1 highly expressed in a variety of tissues including the hematopoietic cells where it influences diverse cellular activities. It maintains normal cellular functions by promoting actin polymerization and interacting with actin-binding proteins.
Biological function summary

The interaction of WIPF1 extends to facilitating cell morphology and movement. It acts as part of a complex by binding to WASP (Wiskott-Aldrich Syndrome Protein) which regulates the building of actin filaments in response to cellular signals. Through its biological actions WIPF1 contributes to processes such as endocytosis phagocytosis and cell migration. These roles are essential for maintaining cell dynamics and structure.

Pathways

The involvement of WIPF1 in the actin regulation pathways is significant. It participates in pathways like the Rho GTPase pathway which controls actin dynamics and the Arp2/3 complex-mediated pathway which promotes branching in actin filaments. The interaction of WIPF1 with proteins such as CDC42 and N-WASP highlights its importance in signal transduction leading to actin assembly and cellular responses.

Mutations or dysfunctions in WIPF1 associate with certain immunodeficiencies such as Wiskott-Aldrich syndrome where defective actin regulation occurs. Aberrations in WIPF1 expression also have associations with cancers particularly those affecting hematopoietic cells. The protein's link with WASP underlines its role in these conditions highlighting the importance of proper actin dynamics for normal immune function and cellular stability.
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Specifications

Form

Liquid

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General info

Function

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.

Sequence similarities

Belongs to the verprolin family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
See full target information WIPF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:7321-7334 PubMed34245091

2021

LncRNA PAXIP1-AS1 fosters the pathogenesis of pulmonary arterial hypertension via ETS1/WIPF1/RhoA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Song,Si Lei,Song Yang,Shang-Jie Wu

Cancer medicine 9:6752-6765 PubMed32725768

2020

Long noncoding RNA HCG18 up-regulates the expression of WIPF1 and YAP/TAZ by inhibiting miR-141-3p in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Wenji Lin,Yangyang Dong,Xinyu Li,Zhibin Lin,Jing Jia,Wenbing Zou,Yu Pan
View all publications
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