Recombinant Human WISP2 protein (Animal Free) is a Human Full Length protein, in the 24 to 250 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M Q L C P T P C T C P W P P P R C P L G V P L V L D G C G C C R V C A R R L G E P C D Q L H V C D A S Q G L V C Q P G A G P G G R G A L C L L A E D D S S C E V N G R L Y R E G E T F Q P H C S I R C R C E D G G F T C V P L C S E D V R L P S W D C P H P R R V E V L G K C C P E W V C G Q G G G L G T Q P L P A Q G P Q F S G L V S S L P P G V P C P E W S T A W G P C S T T C G L G M A T R V S N Q N R F C R L E T Q R R L C L S R P C P P S R G R S P Q N S A F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Alternative names
CT58, CTGFL, WISP2, UNQ228/PRO261, CCN5, CCN family member 5, Connective tissue growth factor-like protein, Connective tissue growth factor-related protein 58, WNT1-inducible-signaling pathway protein 2, CTGF-L, WISP-2
Recommended products
Recombinant Human WISP2 protein (Animal Free) is a Human Full Length protein, in the 24 to 250 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O76076-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in 10 mM acetic acid
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- M Q L C P T P C T C P W P P P R C P L G V P L V L D G C G C C R V C A R R L G E P C D Q L H V C D A S Q G L V C Q P G A G P G G R G A L C L L A E D D S S C E V N G R L Y R E G E T F Q P H C S I R C R C E D G G F T C V P L C S E D V R L P S W D C P H P R R V E V L G K C C P E W V C G Q G G G L G T Q P L P A Q G P Q F S G L V S S L P P G V P C P E W S T A W G P C S T T C G L G M A T R V S N Q N R F C R L E T Q R R L C L S R P C P P S R G R S P Q N S A F
- Accession
- O76076
- Protein length
- Full Length
- Predicted molecular weight
- 24.4 kDa
- Amino acids
- 24 to 250
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
- Sequence similarities
Belongs to the CCN family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WISP2 also known as WNT1-inducible signaling pathway protein 2 or CCN5 is a protein that weighs approximately 29 kDa. This protein belongs to the CCN family which includes connective tissue growth factor and nephroblastoma overexpressed gene. WISP2 expression appears in various human tissues particularly in the liver lung and breast tissues. It is known to engage in cellular processes often impacting cell growth and differentiation due to its role in signaling pathways.
- Biological function summary
Researchers observe WISP2’s significant role in cell proliferation migration and anti-apoptotic activities. It is known to not be part of a larger protein complex. This protein influences cell behavior and works in modulating functions related to the extracellular matrix. This modulation aids cellular restructuring and organization which are essential processes in tissue repair and development.
- Pathways
Studies demonstrate that WISP2 plays a role in the Wnt signaling and PI3K/Akt pathways. The presence of WISP2 in these pathways allows it to control signals that regulate cell growth and survival. Within these pathways WISP2 interacts with several proteins including beta-catenin and AKT1 which help in transmitting signals from cell surface receptors to the DNA in the nucleus influencing gene expression and cellular responses.
- Associated diseases and disorders
WISP2 shows relevance in cancer and fibrosis. Researchers study its expression in breast cancer noting that it impacts tumor development and progression by interacting with other proteins like estrogen receptor-alpha (ERα). Moreover in the context of fibrosis WISP2 may influence fibrotic processes possibly involving connective tissue growth factor (CTGF) which contributes to tissue scarring and thickening through extracellular matrix production.
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1 product image
SDS-PAGE - Recombinant Human WISP2 protein (Animal Free) (ab256117)
SDS-PAGE analysis of ab256117 (1μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions. ab256117 is predicted to have a MW of 24.4 kDa.
4-20% Tris-Glycine gel. Coomassie Blue staining.
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