Recombinant human WNK3 protein (Active)
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Recombinant human WNK3 protein (Active) is a Human Fragment protein, in the 1 to 571 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
KIAA1566, PRKWNK3, WNK3, Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3
- FuncS
Supplier Data
Functional Studies - Recombinant human WNK3 protein (Active) (AB269136)
ab269136 specific activity was determined to be 13 nmol/min/mg in a kinase assay using myelin basic protein (MBP) substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human WNK3 protein (Active) (AB269136)
SDS-PAGE analysis of ab269136.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
WNK3 plays a critical role in cellular ion homeostasis and modulation of ion transporters in response to osmotic changes. It is part of a complex that regulates ion balance within cells affecting cell volume and blood pressure. Through phosphorylation activities WNK3 can activate or inhibit other kinases impacting cellular responses to various stimuli. These regulatory actions are significant for maintaining proper electrolyte balance and fluid dynamics in the body.
Pathways
WNK3 is involved in regulating the WNK-SPAK/OSR1 pathway which is essential in controlling ion transporter activities in cells. This pathway fine-tunes the activity of the NCC and NKCC transporters. WNK3 is closely connected with other kinases like WNK1 and WNK4 with all playing common roles in blood pressure regulation and ion transport. The coordination between these kinases ensures precise cellular responses to changes in osmolarity and extracellular signals.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress (PubMed : 16275911, PubMed : 16275913, PubMed : 16501604, PubMed : 22989884, PubMed : 36318922). WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation (PubMed : 36318922). The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (PubMed : 22989884). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed : 16275911, PubMed : 16275913). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux (PubMed : 16275911, PubMed : 16275913, PubMed : 16357011, PubMed : 19470686, PubMed : 21613606). Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC (PubMed : 17975670). May also phosphorylate NEDD4L (PubMed : 20525693). Also acts as a scaffold protein independently of its protein kinase activity : negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9 (PubMed : 16357011, PubMed : 17673510). Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway (PubMed : 18768590).
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Post-translational modifications
Autophosphorylated at Ser-304 and Ser-308, promoting its activity (PubMed:33439774). Phosphorylation at Thr-541 prevents interaction with KLHL3 and subsequent ubiquitination and degradation by the BCR(KLHL3) complex (Probable) (PubMed:35179207).. Ubiquitinated by the BCR(KLHL2) complex, leading to its degradation (PubMed:23838290). Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation (PubMed:35179207).
Target data
Product promise
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