AB84080

Recombinant human Wnt1 protein (Active)

Bioactive
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(10 Publications)

Recombinant human Wnt1 protein (Active) is a Human Full Length protein, in the 28 to 370 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.

View Alternative Names

INT1, WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Applications

FuncS, HPLC, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The ED₅₀ was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells.

The expected ED₅₀ for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Accession

P04628

Animal free

Carrier free

Species

Human

Reconstitution

Reconstitute in water

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Sequence info

[{"sequence":"ANSSGRWWGIVNVASSTNLLTDSKSLQLVLEPSLQLLSRKQRRLIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPTAPGPHLFGKIVNRGCRETAFIFAITSAGVTHSVARSCSEGSIESCTCDYRRRGPGGPDWHWGGCSDNIDFGRLFGREFVDSGEKGRDLRFLMNLHNNEAGRTTVFSEMRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGSNRASRAELLRLEPEDPAHKPPSPHDLVYFEKSPNFCTYSGRLGTAGTAGRACNSSSPALDGCELLCCGRGHRTRTQRVTERCNCTFHWCCHVSCRNCTHTRVLHECL","proteinLength":"Full Length","predictedMolecularWeight":"38.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":370,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P04628","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt1 also known as Int-1 is a member of the Wnt gene family and is known for its role in encoding a protein that is involved in signaling pathways inside the cell. This protein weighs approximately 41 kDa and is expressed in various tissues including the brain and lungs. It functions by binding to receptors on the cell surface activating intracellular signaling pathways that govern cellular processes like proliferation and differentiation.

Biological function summary

Wnt1 plays a significant role in embryonic development. It is an integral component of the Wnt signaling complex which regulates important aspects such as cell fate determination patterning during embryogenesis and organogenesis. The protein's activity ensures that cells behave in a coordinated manner allowing for proper tissue development and growth.

Pathways

Wnt1 is essential in the canonical Wnt/β-catenin signaling pathway. This pathway involves the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus where it influences gene transcription. It also plays a role in the non-canonical Wnt pathways such as the planar cell polarity pathway but its primary activity is within the canonical one. Proteins like Frizzled and Dishevelled are closely related through these pathways facilitating the transduction of signals initiated by Wnt1.

Wnt1 has been linked to cancers including breast and colorectal cancer due to its role in regulating cell proliferation and survival. Dysregulation of Wnt1 signaling can lead to poor control over cell growth contributing to oncogenesis. Wnt1 is also associated with bone density disorders as it critically affects bone formation and remodeling. In these contexts proteins such as LRP5/6 and β-catenin interact with Wnt1 mediating its effects on disease progression.

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Specifications

Form

Lyophilized

Additional notes

>98% HPLC.

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General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed : 23499309, PubMed : 23656646, PubMed : 26902720, PubMed : 28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed : 23499309, PubMed : 23656646).

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information WNT1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

BMC molecular and cell biology 21:80 PubMed33167868

2020

Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Chen,Jiuyang Jiao,Youyuan Wang,Zhihui Mai,Jing Ren,Sijie He,Xiaolan Li,Zheng Chen

Neural regeneration research 14:1013-1024 PubMed30762013

2019

Neuroprotection mediated by the Wnt/Frizzled signaling pathway in early brain injury induced by subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,De-Jun Bao,Bin Xu,Chuan-Dong Cheng,Yong-Fei Dong,Xiang-Pin Wei,Chao-Shi Niu

American journal of physiology. Lung cellular and 306:L645-60 PubMed24487391

2014

Sox2 modulates Lef-1 expression during airway submucosal gland development.

Applications

Unspecified application

Species

Mouse

Weiliang Xie,Thomas J Lynch,Xiaoming Liu,Scott R Tyler,Shuyang Yu,Xinyuan Zhou,Meihui Luo,David M Kusner,Xingshen Sun,Yaling Yi,Yulong Zhang,Michael J Goodheart,Kalpaj R Parekh,James M Wells,Hai-Hui Xue,Larysa H Pevny,John F Engelhardt

Journal of cell science 126:2877-89 PubMed23613467

2013

The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Wang-Yu Cai,Tong-Zhen Wei,Qi-Cong Luo,Qiu-Wan Wu,Qing-Feng Liu,Meng Yang,Guo-Dong Ye,Jia-Fa Wu,Yuan-Yuan Chen,Guang-Bin Sun,Yun-Jia Liu,Wen-Xiu Zhao,Zhi-Ming Zhang,Bo-An Li

Nature 487:500-4 PubMed22763439

2012

Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Ravid Straussman,Teppei Morikawa,Kevin Shee,Michal Barzily-Rokni,Zhi Rong Qian,Jinyan Du,Ashli Davis,Margaret M Mongare,Joshua Gould,Dennie T Frederick,Zachary A Cooper,Paul B Chapman,David B Solit,Antoni Ribas,Roger S Lo,Keith T Flaherty,Shuji Ogino,Jennifer A Wargo,Todd R Golub

The EMBO journal 31:429-42 PubMed22085926

2011

Wnt1/βcatenin injury response activates the epicardium and cardiac fibroblasts to promote cardiac repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jinzhu Duan,Costin Gherghe,Dianxin Liu,Eric Hamlett,Luxman Srikantha,Laurel Rodgers,Jenna N Regan,Mauricio Rojas,Monte Willis,Andrew Leask,Mark Majesky,Arjun Deb

Stem cells (Dayton, Ohio) 27:1130-41 PubMed19418460

2009

Endogenous Wnt signaling maintains neural progenitor cell potency.

Applications

Unspecified application

Species

Unspecified reactive species

Eric M Wexler,Andres Paucer,Harley I Kornblum,Theodore D Palmer,Theodore D Plamer,Daniel H Geschwind

Nature neuroscience 12:125-31 PubMed19122665

2009

Wnt antagonism of Shh facilitates midbrain floor plate neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Milan Joksimovic,Beth A Yun,Raja Kittappa,Angela M Anderegg,Wendy W Chang,Makoto M Taketo,Ronald D G McKay,Rajeshwar B Awatramani

The Journal of biological chemistry 282:16811-9 PubMed17412694

2007

Phosphorylation of p68 RNA helicase plays a role in platelet-derived growth factor-induced cell proliferation by up-regulating cyclin D1 and c-Myc expression.

Applications

Unspecified application

Species

Unspecified reactive species

Liuqing Yang,Chunru Lin,Shumin Zhao,Haizhen Wang,Zhi-Ren Liu

Cancer research 67:57-65 PubMed17210683

2007

Role for amplification and expression of glypican-5 in rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Williamson,Joanna Selfe,Tony Gordon,Yong-Jie Lu,Kathy Pritchard-Jones,Kasumi Murai,Phil Jones,Paul Workman,Janet Shipley

View all publications

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Product promise

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Recombinant human Wnt1 protein (Active)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

Complementary Products

Reagents, Controls & Accessories

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (10)

Product promise