Recombinant human Wnt1 protein (Active) is a Human Full Length protein, in the 28 to 370 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, FuncS.
A N S S G R W W G I V N V A S S T N L L T D S K S L Q L V L E P S L Q L L S R K Q R R L I R Q N P G I L H S V S G G L Q S A V R E C K W Q F R N R R W N C P T A P G P H L F G K I V N R G C R E T A F I F A I T S A G V T H S V A R S C S E G S I E S C T C D Y R R R G P G G P D W H W G G C S D N I D F G R L F G R E F V D S G E K G R D L R F L M N L H N N E A G R T T V F S E M R Q E C K C H G M S G S C T V R T C W M R L P T L R A V G D V L R D R F D G A S R V L Y G N R G S N R A S R A E L L R L E P E D P A H K P P S P H D L V Y F E K S P N F C T Y S G R L G T A G T A G R A C N S S S P A L D G C E L L C C G R G H R T R T Q R V T E R C N C T F H W C C H V S C R N C T H T R V L H E C L
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed:23499309, PubMed:23656646, PubMed:26902720, PubMed:28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed:23499309, PubMed:23656646).
INT1, WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
Recombinant human Wnt1 protein (Active) is a Human Full Length protein, in the 28 to 370 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, FuncS.
The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
>98% HPLC.
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed:23499309, PubMed:23656646, PubMed:26902720, PubMed:28528193). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed:23499309, PubMed:23656646).
Belongs to the Wnt family.
Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Wnt1 also known as Int-1 is a member of the Wnt gene family and is known for its role in encoding a protein that is involved in signaling pathways inside the cell. This protein weighs approximately 41 kDa and is expressed in various tissues including the brain and lungs. It functions by binding to receptors on the cell surface activating intracellular signaling pathways that govern cellular processes like proliferation and differentiation.
Wnt1 plays a significant role in embryonic development. It is an integral component of the Wnt signaling complex which regulates important aspects such as cell fate determination patterning during embryogenesis and organogenesis. The protein's activity ensures that cells behave in a coordinated manner allowing for proper tissue development and growth.
Wnt1 is essential in the canonical Wnt/β-catenin signaling pathway. This pathway involves the stabilization and accumulation of β-catenin in the cytoplasm and its subsequent translocation into the nucleus where it influences gene transcription. It also plays a role in the non-canonical Wnt pathways such as the planar cell polarity pathway but its primary activity is within the canonical one. Proteins like Frizzled and Dishevelled are closely related through these pathways facilitating the transduction of signals initiated by Wnt1.
Wnt1 has been linked to cancers including breast and colorectal cancer due to its role in regulating cell proliferation and survival. Dysregulation of Wnt1 signaling can lead to poor control over cell growth contributing to oncogenesis. Wnt1 is also associated with bone density disorders as it critically affects bone formation and remodeling. In these contexts proteins such as LRP5/6 and β-catenin interact with Wnt1 mediating its effects on disease progression.
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