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Recombinant Human Wnt5a protein is a Human Full Length protein, in the 62 to 380 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.

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Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M I I G A Q P L C S Q L A G L S Q G Q K K L C H L Y Q D H M Q Y I G E G A K T G I K E C Q Y Q F R H R R W N C S T V D N T S V F G R V M Q I G S R E T A F T Y A V S A A G V V N A M S R A C R E G E L S T C G C S R A A R P K D L P R D W L W G G C G D N I D Y G Y R F A K E F V D A R E R E R I H A K G S Y E S A R I L M N L H N N E A G R R T V Y N L A D V A C K C H G V S G S C S L K T C W L Q L A D F R K V G D A L K E K Y D S A A A M R L N S R G K L V Q V N S R F N S P T T Q D L V Y I D P S P D Y C V R N E S T G S L G T Q G R L C N K T S E G M D G C E L M C C G R G Y D Q F K T V Q T E R C H C K F H W C C Y V K C K K C T E I V D Q F V C K L E H H H H H

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).

Alternative names

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Recombinant Human Wnt5a protein is a Human Full Length protein, in the 62 to 380 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
P41221-1
Animal free

No

Species

Human

Reconstitution

Reconstitute in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.

Concentration
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Storage buffer

Constituents: 0.3% Acetic acid

Sequence info

Amino acid sequence

M I I G A Q P L C S Q L A G L S Q G Q K K L C H L Y Q D H M Q Y I G E G A K T G I K E C Q Y Q F R H R R W N C S T V D N T S V F G R V M Q I G S R E T A F T Y A V S A A G V V N A M S R A C R E G E L S T C G C S R A A R P K D L P R D W L W G G C G D N I D Y G Y R F A K E F V D A R E R E R I H A K G S Y E S A R I L M N L H N N E A G R R T V Y N L A D V A C K C H G V S G S C S L K T C W L Q L A D F R K V G D A L K E K Y D S A A A M R L N S R G K L V Q V N S R F N S P T T Q D L V Y I D P S P D Y C V R N E S T G S L G T Q G R L C N K T S E G M D G C E L M C C G R G Y D Q F K T V Q T E R C H C K F H W C C Y V K C K K C T E I V D Q F V C K L E H H H H H

Accession

P41221

Protein length

Full Length

Predicted molecular weight

36.9 kDa

Amino acids

62 to 380

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

Additional notes

Purified by Nickel Sepharose HighTrap GEEndotoxin level: < 0.1 ng per µg of Wnt5a

General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Glycosylation is necessary for secretion but not for activity.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

No direct biological data available at the moment but ab204627 binds to immobilized Human sROR2 in a functional ELISA.

Supplementary info

Biological function summary

Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.

Activity summary

Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.

Pathways

Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.

Associated diseases and disorders

Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.

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1 product image

  • SDS-PAGE - Recombinant Human Wnt5a protein (ab204627), expandable thumbnail

    SDS-PAGE - Recombinant Human Wnt5a protein (ab204627)

    15% SDS-PAGE analysis of ab204627 under reducing condtions and stained with Coomassie blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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