Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Recombinant Human Wnt5a protein is a Human Full Length protein, in the 62 to 380 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
>95% SDS-PAGE
Escherichia coli
His tag C-Terminus
SDS-PAGE
No
M I I G A Q P L C S Q L A G L S Q G Q K K L C H L Y Q D H M Q Y I G E G A K T G I K E C Q Y Q F R H R R W N C S T V D N T S V F G R V M Q I G S R E T A F T Y A V S A A G V V N A M S R A C R E G E L S T C G C S R A A R P K D L P R D W L W G G C G D N I D Y G Y R F A K E F V D A R E R E R I H A K G S Y E S A R I L M N L H N N E A G R R T V Y N L A D V A C K C H G V S G S C S L K T C W L Q L A D F R K V G D A L K E K Y D S A A A M R L N S R G K L V Q V N S R F N S P T T Q D L V Y I D P S P D Y C V R N E S T G S L G T Q G R L C N K T S E G M D G C E L M C C G R G Y D Q F K T V Q T E R C H C K F H W C C Y V K C K K C T E I V D Q F V C K L E H H H H H
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
Protein Wnt-5a, WNT5A
Recombinant Human Wnt5a protein is a Human Full Length protein, in the 62 to 380 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Protein Wnt-5a, WNT5A
>95% SDS-PAGE
Escherichia coli
His tag C-Terminus
SDS-PAGE
No
No
Human
Reconstitute in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.
Constituents: 0.3% Acetic acid
M I I G A Q P L C S Q L A G L S Q G Q K K L C H L Y Q D H M Q Y I G E G A K T G I K E C Q Y Q F R H R R W N C S T V D N T S V F G R V M Q I G S R E T A F T Y A V S A A G V V N A M S R A C R E G E L S T C G C S R A A R P K D L P R D W L W G G C G D N I D Y G Y R F A K E F V D A R E R E R I H A K G S Y E S A R I L M N L H N N E A G R R T V Y N L A D V A C K C H G V S G S C S L K T C W L Q L A D F R K V G D A L K E K Y D S A A A M R L N S R G K L V Q V N S R F N S P T T Q D L V Y I D P S P D Y C V R N E S T G S L G T Q G R L C N K T S E G M D G C E L M C C G R G Y D Q F K T V Q T E R C H C K F H W C C Y V K C K K C T E I V D Q F V C K L E H H H H H
Full Length
36.9 kDa
62 to 380
Recombinant
His tag C-Terminus
Lyophilized
Purified by Nickel Sepharose HighTrap GEEndotoxin level: < 0.1 ng per µg of Wnt5a
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
Belongs to the Wnt family.
Glycosylation is necessary for secretion but not for activity.
Blue Ice
-20°C
Avoid freeze / thaw cycle
No direct biological data available at the moment but ab204627 binds to immobilized Human sROR2 in a functional ELISA.
Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.
Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.
Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.
Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
15% SDS-PAGE analysis of ab204627 under reducing condtions and stained with Coomassie blue.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com