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AB116171

Recombinant human Wnt7a protein

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(5 Publications)

Recombinant human Wnt7a protein is a Human Full Length protein, in the 32 to 349 aa range, expressed in HEK 293 cells, with >80%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Protein Wnt-7a, WNT7A

Key facts

Purity

>80% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Determined by its ability to decrease alkaline phosphatase activity in CCL-226 cells when treated with 25 ng/ml of Murine Wnt-3a.

Accession

O00755

Animal free

No

Carrier free

No

Species

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGLDECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQYHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCTTKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIKKPLSYRKPMDTDLVYIEKSPNYCEEDPVTGSVGTQGRACNKTAPQASGCDLMCCGRGYNTHQYARVWQCNCKFHWCCYVKCNTCSERTEMYTCK","proteinLength":"Full Length","predictedMolecularWeight":"35.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":349,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O00755","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt7a also known as Wingless-type MMTV integration site family member 7A is a protein involved in various cellular processes. It has a molecular mass of approximately 39 kDa and is expressed in several tissues including the brain lungs and kidneys. Mechanically Wnt7a acts as a signaling molecule that binds to frizzled receptors on cell surfaces initiating downstream signaling cascades that result in the transcriptional regulation of target genes.
Biological function summary

This protein plays a critical role in the regulation of developmental processes including cell proliferation differentiation and migration. Wnt7a is part of the Wnt signaling pathway which is a complex network of proteins known for its involvement in embryonic development and regulation of cell fate decisions. The pathway contributes to the proper formation of tissues and organs and maintains cellular homeostasis.

Pathways

Wnt7a is significantly involved in the canonical Wnt/β-catenin signaling pathway as well as the planar cell polarity pathway. In the canonical pathway Wnt7a influences the stabilization and translocation of β-catenin to the nucleus promoting transcription of target genes. It also interacts with other Wnt proteins like Wnt3a and different frizzled receptors playing roles in various cell signaling events and modulating cellular communication.

Wnt7a is linked to limb malformation disorders such as Fuhrmann syndrome and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome. These conditions arise from mutations that affect the function of Wnt7a disrupting normal limb development. Additionally aberrant Wnt7a signaling has implications in cancer particularly in the modulation of tumor microenvironment and metastasis where altered interactions with proteins like β-catenin can contribute to oncogenic processes.
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Specifications

Form

Lyophilized

Additional notes

The purity of ab116171 is greater than 80% by SDS-PAGE gel and HPLC analyses.

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General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed : 16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

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Product protocols

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed : 16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).
See full target information WNT7A
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3753 PubMed40307257

2025

Divergent actions of physiological and pathological amyloid-β on synapses in live human brain slice cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Robert I McGeachan,Soraya Meftah,Lewis W Taylor,James H Catterson,Danilo Negro,Calum Bonthron,Kristján Holt,Jane Tulloch,Jamie L Rose,Francesco Gobbo,Ya Yin Chang,Jamie Elliott,Lauren McLay,Declan King,Imran Liaquat,Tara L Spires-Jones,Sam A Booker,Paul M Brennan,Claire S Durrant

Cancer science 115:3013-3025 PubMed38923741

2024

RECK/GPR124-driven WNT signaling in pancreatic and gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Yu,Susumu Kohno,Dominic Chih-Cheng Voon,Nada Hamdy Hussein,Yuanyuan Zhang,Joji Nakayama,Yujiro Takegami,Chiaki Takahashi

Nature communications 15:3580 PubMed38678032

2024

The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Hui Chang,Feng Liu,Stefania Militi,Svenja Hester,Reshma Nibhani,Siwei Deng,James Dunford,Aniko Rendek,Zahir Soonawalla,Roman Fischer,Udo Oppermann,Siim Pauklin

Acta neuropathologica 147:7 PubMed38175261

2024

p-tau Ser356 is associated with Alzheimer's disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003.

Applications

Unspecified application

Species

Unspecified reactive species

Lewis W Taylor,Elizabeth M Simzer,Claire Pimblett,Oscar T T Lacey-Solymar,Robert I McGeachan,Soraya Meftah,Jamie L Rose,Maxwell P Spires-Jones,Kristján Holt,James H Catterson,Henner Koch,Imran Liaquat,Jonathan H Clarke,John Skidmore,Colin Smith,Sam A Booker,Paul M Brennan,Tara L Spires-Jones,Claire S Durrant

Nature communications 7:10354 PubMed26838601

2016

Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.

Applications

Unspecified application

Species

Unspecified reactive species

K Long,L Moss,L Laursen,L Boulter,C Ffrench-Constant
View all publications
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