Recombinant Human Wnt7a protein (denatured) is a Human Full Length protein, in the 32 to 349 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S L G A S I I C N K I P G L A P R Q R A I C Q S R P D A I I V I G E G S Q M G L D E C Q F Q F R N G R W N C S A L G E R T V F G K E L K V G S R E A A F T Y A I I A A G V A H A I T A A C T Q G N L S D C G C D K E K Q G Q Y H R D E G W K W G G C S A D I R Y G I G F A K V F V D A R E I K Q N A R T L M N L H N N E A G R K I L E E N M K L E C K C H G V S G S C T T K T C W T T L P Q F R E L G Y V L K D K Y N E A V H V E P V R A S R N K R P T F L K I K K P L S Y R K P M D T D L V Y I E K S P N Y C E E D P V T G S V G T Q G R A C N K T A P Q A S G C D L M C C G R G Y N T H Q Y A R V W Q C N C K F H W C C Y V K C N T C S E R T E M Y T C K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).
Alternative names
Protein Wnt-7a, WNT7A
Recommended products
Recombinant Human Wnt7a protein (denatured) is a Human Full Length protein, in the 32 to 349 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O00755-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S L G A S I I C N K I P G L A P R Q R A I C Q S R P D A I I V I G E G S Q M G L D E C Q F Q F R N G R W N C S A L G E R T V F G K E L K V G S R E A A F T Y A I I A A G V A H A I T A A C T Q G N L S D C G C D K E K Q G Q Y H R D E G W K W G G C S A D I R Y G I G F A K V F V D A R E I K Q N A R T L M N L H N N E A G R K I L E E N M K L E C K C H G V S G S C T T K T C W T T L P Q F R E L G Y V L K D K Y N E A V H V E P V R A S R N K R P T F L K I K K P L S Y R K P M D T D L V Y I E K S P N Y C E E D P V T G S V G T Q G R A C N K T A P Q A S G C D L M C C G R G Y N T H Q Y A R V W Q C N C K F H W C C Y V K C N T C S E R T E M Y T C K
- Accession
- O00755
- Protein length
- Full Length
- Predicted molecular weight
- 38 kDa
- Amino acids
- 32 to 349
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).
- Sequence similarities
Belongs to the Wnt family.
- Post-translational modifications
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt7a also known as Wingless-type MMTV integration site family member 7A is a protein involved in various cellular processes. It has a molecular mass of approximately 39 kDa and is expressed in several tissues including the brain lungs and kidneys. Mechanically Wnt7a acts as a signaling molecule that binds to frizzled receptors on cell surfaces initiating downstream signaling cascades that result in the transcriptional regulation of target genes.
- Biological function summary
This protein plays a critical role in the regulation of developmental processes including cell proliferation differentiation and migration. Wnt7a is part of the Wnt signaling pathway which is a complex network of proteins known for its involvement in embryonic development and regulation of cell fate decisions. The pathway contributes to the proper formation of tissues and organs and maintains cellular homeostasis.
- Pathways
Wnt7a is significantly involved in the canonical Wnt/β-catenin signaling pathway as well as the planar cell polarity pathway. In the canonical pathway Wnt7a influences the stabilization and translocation of β-catenin to the nucleus promoting transcription of target genes. It also interacts with other Wnt proteins like Wnt3a and different frizzled receptors playing roles in various cell signaling events and modulating cellular communication.
- Associated diseases and disorders
Wnt7a is linked to limb malformation disorders such as Fuhrmann syndrome and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome. These conditions arise from mutations that affect the function of Wnt7a disrupting normal limb development. Additionally aberrant Wnt7a signaling has implications in cancer particularly in the modulation of tumor microenvironment and metastasis where altered interactions with proteins like β-catenin can contribute to oncogenic processes.
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1 product image
SDS-PAGE - Recombinant Human Wnt7a protein (denatured) (ab171487)
15% SDS-PAGE analysis of 3µg ab171487.
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