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AB289780

Recombinant Human Wnt7b protein

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(1 Publication)

Recombinant Human Wnt7b protein is a Human Full Length protein, in the 25 to 349 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Protein Wnt-7b, WNT7B

1 Images
SDS-PAGE - Recombinant Human Wnt7b protein (AB289780)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Wnt7b protein (AB289780)

SDS-PAGE analysis of ab289780

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus SUMO tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P56706

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.12% Tris, 0.037% Ethylenediaminetetraacetic acid, disodium, dihydrate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ALSSVVALGANIICNKIPGLAPRQRAICQSRPDAIIVIGEGAQMGINECQYQFRFGRWNCSALGEKTVFGQELRVGSREAAFTYAITAAGVAHAVTAACSQGNLSNCGCDREKQGYYNQAEGWKWGGCSADVRYGIDFSRRFVDAREIKKNARRLMNLHNNEAGRKVLEDRMQLECKCHGVSGSCTTKTCWTTLPKFREVGHLLKEKYNAAVQVEVVRASRLRQPTFLRIKQLRSYQKPMETDLVYIEKSPNYCEEDAATGSVGTQGRLCNRTSPGADGCDTMCCGRGYNTHQYTKVWQCNCKFHWCCFVKCNTCSERTEVFTCK","proteinLength":"Full Length","predictedMolecularWeight":"56.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":349,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P56706","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt7b also known as Wingless-type MMTV integration site family member 7B is a secreted glycoprotein involved in cell signaling. It has a molecular mass of around 39 kDa. Wnt7b is expressed in various tissues including the brain lung and reproductive organs. This protein serves an important role in mediating intercellular communication by binding to receptors on the cell surface which activates downstream signaling cascades.
Biological function summary

Wnt7b regulates many processes such as cell proliferation differentiation and migration. Part of the Wnt signaling complex it acts as a ligand that binds to Frizzled and LRP5/6 receptor complexes. This initiates a signaling cascade that influences gene expression thereby impacting cellular fate decisions. Wnt7b has significant roles during embryonic development and tissue homeostasis in adults.

Pathways

Several major pathways rely on Wnt7b for proper function including the canonical Wnt/β-catenin pathway and the non-canonical planar cell polarity pathway. Within these pathways Wnt7b interacts with several proteins such as β-catenin and Dishevelled to modulate cellular responses. This regulatory influence is key for maintaining normal physiological processes where the balance of pathway activation dictates the outcome of cellular events.

Many studies link Wnt7b to conditions like lung fibrosis and cancer. In lung fibrosis Wnt7b signaling contributes to fibroblast activation and extracellular matrix production processes commonly exacerbated in this disease. Meanwhile in some cancers aberrations in the Wnt/β-catenin pathway involving Wnt7b can lead to uncontrolled cell growth and tumor development with overexpression resulting in enhanced proliferation. Therefore Wnt7b and closely associated proteins like β-catenin become potential targets for therapeutic intervention in these conditions.
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Specifications

Form

Lyophilized

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General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed : 30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314).

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

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Product protocols

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed : 30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314).
See full target information WNT7B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3580 PubMed38678032

2024

The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Hui Chang,Feng Liu,Stefania Militi,Svenja Hester,Reshma Nibhani,Siwei Deng,James Dunford,Aniko Rendek,Zahir Soonawalla,Roman Fischer,Udo Oppermann,Siim Pauklin
View all publications
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Product promise

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