Recombinant Human Wnt7b protein is a Human Full Length protein, in the 25 to 349 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A L S S V V A L G A N I I C N K I P G L A P R Q R A I C Q S R P D A I I V I G E G A Q M G I N E C Q Y Q F R F G R W N C S A L G E K T V F G Q E L R V G S R E A A F T Y A I T A A G V A H A V T A A C S Q G N L S N C G C D R E K Q G Y Y N Q A E G W K W G G C S A D V R Y G I D F S R R F V D A R E I K K N A R R L M N L H N N E A G R K V L E D R M Q L E C K C H G V S G S C T T K T C W T T L P K F R E V G H L L K E K Y N A A V Q V E V V R A S R L R Q P T F L R I K Q L R S Y Q K P M E T D L V Y I E K S P N Y C E E D A A T G S V G T Q G R L C N R T S P G A D G C D T M C C G R G Y N T H Q Y T K V W Q C N C K F H W C C F V K C N T C S E R T E V F T C K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314).
Alternative names
Protein Wnt-7b, WNT7B
Recommended products
Recombinant Human Wnt7b protein is a Human Full Length protein, in the 25 to 349 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P56706-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.12% Tris, 0.037% Ethylenediaminetetraacetic acid, disodium, dihydrate
Sequence info
Amino acid sequence
- A L S S V V A L G A N I I C N K I P G L A P R Q R A I C Q S R P D A I I V I G E G A Q M G I N E C Q Y Q F R F G R W N C S A L G E K T V F G Q E L R V G S R E A A F T Y A I T A A G V A H A V T A A C S Q G N L S N C G C D R E K Q G Y Y N Q A E G W K W G G C S A D V R Y G I D F S R R F V D A R E I K K N A R R L M N L H N N E A G R K V L E D R M Q L E C K C H G V S G S C T T K T C W T T L P K F R E V G H L L K E K Y N A A V Q V E V V R A S R L R Q P T F L R I K Q L R S Y Q K P M E T D L V Y I E K S P N Y C E E D A A T G S V G T Q G R L C N R T S P G A D G C D T M C C G R G Y N T H Q Y T K V W Q C N C K F H W C C F V K C N T C S E R T E V F T C K
- Accession
- P56706
- Protein length
- Full Length
- Predicted molecular weight
- 56.3 kDa
- Amino acids
- 25 to 349
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314).
- Sequence similarities
Belongs to the Wnt family.
- Post-translational modifications
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt7b also known as Wingless-type MMTV integration site family member 7B is a secreted glycoprotein involved in cell signaling. It has a molecular mass of around 39 kDa. Wnt7b is expressed in various tissues including the brain lung and reproductive organs. This protein serves an important role in mediating intercellular communication by binding to receptors on the cell surface which activates downstream signaling cascades.
- Biological function summary
Wnt7b regulates many processes such as cell proliferation differentiation and migration. Part of the Wnt signaling complex it acts as a ligand that binds to Frizzled and LRP5/6 receptor complexes. This initiates a signaling cascade that influences gene expression thereby impacting cellular fate decisions. Wnt7b has significant roles during embryonic development and tissue homeostasis in adults.
- Pathways
Several major pathways rely on Wnt7b for proper function including the canonical Wnt/β-catenin pathway and the non-canonical planar cell polarity pathway. Within these pathways Wnt7b interacts with several proteins such as β-catenin and Dishevelled to modulate cellular responses. This regulatory influence is key for maintaining normal physiological processes where the balance of pathway activation dictates the outcome of cellular events.
- Associated diseases and disorders
Many studies link Wnt7b to conditions like lung fibrosis and cancer. In lung fibrosis Wnt7b signaling contributes to fibroblast activation and extracellular matrix production processes commonly exacerbated in this disease. Meanwhile in some cancers aberrations in the Wnt/β-catenin pathway involving Wnt7b can lead to uncontrolled cell growth and tumor development with overexpression resulting in enhanced proliferation. Therefore Wnt7b and closely associated proteins like β-catenin become potential targets for therapeutic intervention in these conditions.
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SDS-PAGE - Recombinant Human Wnt7b protein (ab289780)
SDS-PAGE analysis of ab289780
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