Recombinant Human Wnt9a protein is a Human Full Length protein, in the 1 to 365 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
Amino acid sequence
M L D G S P L A R W L A A A F G L T L L L A A L R P S A A Y F G L T G S E P L T I L P L T L E P E A A A Q A H Y K A C D R L K L E R K Q R R M C R R D P G V A E T L V E A V S M S A L E C Q F Q F R F E R W N C T L E G R Y R A S L L K R G F K E T A F L Y A I S S A G L T H A L A K A C S A G R M E R C T C D E A P D L E N R E A W Q W G G C G D N L K Y S S K F V K E F L G R R S S K D L R A R V D F H N N L V G V K V I K A G V E T T C K C H G V S G S C T V R T C W R Q L A P F H E V G K H L K H K Y E T A L K V G S T T N E A A G E A G A I S P P R G R A S G A G G S D P L P R T P E L V H L D D S P S F C L A G R F S P G T A G R R C H R E K N C E S I C C G R G H N T Q S R V V T R P C Q C Q V R W C C Y V E C R Q C T Q R E E V Y T C K G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.
Alternative names
WNT14, WNT14, WNT9A, Protein Wnt-9a, Protein Wnt-14
Recommended products
Recombinant Human Wnt9a protein is a Human Full Length protein, in the 1 to 365 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
- Accession
- O14904-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M L D G S P L A R W L A A A F G L T L L L A A L R P S A A Y F G L T G S E P L T I L P L T L E P E A A A Q A H Y K A C D R L K L E R K Q R R M C R R D P G V A E T L V E A V S M S A L E C Q F Q F R F E R W N C T L E G R Y R A S L L K R G F K E T A F L Y A I S S A G L T H A L A K A C S A G R M E R C T C D E A P D L E N R E A W Q W G G C G D N L K Y S S K F V K E F L G R R S S K D L R A R V D F H N N L V G V K V I K A G V E T T C K C H G V S G S C T V R T C W R Q L A P F H E V G K H L K H K Y E T A L K V G S T T N E A A G E A G A I S P P R G R A S G A G G S D P L P R T P E L V H L D D S P S F C L A G R F S P G T A G R R C H R E K N C E S I C C G R G H N T Q S R V V T R P C Q C Q V R W C C Y V E C R Q C T Q R E E V Y T C K G
- Accession
- Protein length
Full Length
- Amino acids
1 to 365
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.
- Sequence similarities
Belongs to the Wnt family.
- Post-translational modifications
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt9a also known as Wingless-related integration site 9A is a secreted glycoprotein with a molecular mass of approximately 39 kDa. This protein forms part of the Wnt family which comprises signaling molecules involved in various developmental processes. Wnt9a is expressed in several tissues including the brain kidneys and skeletal tissues during embryonic development but it can also be found in adult tissues. Its expression pattern indicates a role in spatial and temporal regulation during cell growth and differentiation.
- Biological function summary
Wnt9a functions in the regulation of cell proliferation migration and fate determination. It operates as part of larger protein complexes that include other Wnt family members and co-receptors such as Frizzled receptors. Wnt9a plays a significant role in osteoblast differentiation and skeletal development. It also contributes to the patterning of mesenchymal cells into chondrocytes during skeletal morphogenesis. These biological activities highlight its essential function in both developmental stages and tissue maintenance.
- Pathways
Wnt9a acts through the canonical Wnt/β-catenin signaling pathway and is also involved in the non-canonical Wnt signaling pathway. Within these pathways it interacts with LRP5/6 receptors and related proteins like Dishevelled (Dvl) and Axin. The canonical pathway promotes gene transcription activation whereas the non-canonical pathway influences cell movements and organization. These interactions highlight Wnt9a’s role in the broader signaling cascades important for embryogenesis and homeostasis.
- Associated diseases and disorders
Wnt9a is associated with skeletal malformations such as Robinow syndrome and bone density disorders. Wnt9a mutations can disrupt normal skeletal development leading to abnormal growth and bone formation. In this context interactions with other proteins like BMP4 and Sox9 become disrupted contributing to the pathogenesis of these conditions. Studies continue to explore the therapeutic potential of targeting Wnt9a pathways for treating these skeletal disorders.
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1 product image
SDS-PAGE - Recombinant Human Wnt9a protein (ab159814)
ab159814 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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