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AB245815

Recombinant human Wnt9b protein (Active)

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Recombinant human Wnt9b protein (Active) is a Human Full Length protein, in the 23 to 357 aa range, expressed in CHO cells, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS, HPLC, SDS-PAGE.

View Alternative Names

WNT14B, WNT15, UNQ6973/PRO21956, WNT9B, Protein Wnt-9b, Protein Wnt-14b, Protein Wnt-15

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Determined by its ability to induce alkaline phosphatase production by CCL-226 cells.

Accession

O14905

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 1.45% Sodium chloride, 0.56% Potassium chloride, 0.375% 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, 0.12% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Due to glycosylation, ab245815 migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.</p>" } } }
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Product details

Due to glycosylation, ab245815 migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.
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Sequence info

[{"sequence":"SYFGLTGREVLTPFPGLGTAAAPAQGGAHLKQCDLLKLSRRQKQLCRREPGLAETLRDAAHLGLLECQFQFRHERWNCSLEGRMGLLKRGFKETAFLYAVSSAALTHTLARACSAGRMERCTCDDSPGLESRQAWQWGVCGDNLKYSTKFLSNFLGSKRGNKDLRARADAHNTHVGIKAVKSGLRTTCKCHGVSGSCAVRTCWKQLSPFRETGQVLKLRYDSAVKVSSATNEALGRLELWAPARQGSLTKGLAPRSGDLVYMEDSPSFCRPSKYSPGTAGRVCSREASCSSLCCGRGYDTQSRLVAFSCHCQVQWCCYVECQQCVQEELVYTCKH","proteinLength":"Full Length","predictedMolecularWeight":"36.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":357,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"O14905","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt9b also known as Wingless-related integration site 9b is a protein important for cell signaling. It is part of the Wnt protein family with a mass of approximately 40 kDa. Scientists detect Wnt9b expression in several tissues including the kidney liver and certain cell types in the developing limb. The Wnt proteins play an important role in developmental processes through signaling pathways.
Biological function summary

Wnt9b impacts critical cellular events by mediating signaling processes. It interacts with other proteins forming complexes that influence gene expression and cell fate determination. Wnt9b significantly contributes to embryonic development particularly in organogenesis such as kidney and limb formation. Researchers have noted its role in maintaining the balance between proliferation and differentiation in those tissues.

Pathways

Current research has placed Wnt9b within the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. These pathways involve important proteins like β-catenin in the canonical pathway which affects transcriptional regulation. Wnt9b also interacts with Frizzled receptors modulating downstream signaling effects. These pathways are essential in regulating cellular functions such as stem cell pluripotency and cellular differentiation.

Scientists have linked Wnt9b to developmental disorders and cancer. In kidney development mutations or altered expression can lead to renal dysplasia or agenesis disorders connected with other pathways and factors such as BMP4. In cancer biology aberrant Wnt9b signaling correlates with tumorigenesis particularly in hepatocellular carcinoma where it may interact with proteins like AXIN2 leading to altered cell cycle progression and proliferation. Understanding Wnt9b's involvement in these conditions offers potential for therapeutic targets.
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Specifications

Form

Lyophilized

Additional notes

At least 95% by HPLC analysis.

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General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development (By similarity).

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

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Product protocols

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development (By similarity).
See full target information WNT9B
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