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AB86687

Recombinant Human WWOX protein (His tag)

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Recombinant Human WWOX protein (His tag) is a Human Fragment protein, in the 1 to 234 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

FOR, SDR41C1, WOX1, WWOX, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductase, Short chain dehydrogenase/reductase family 41C member 1

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SDS-PAGE - Recombinant Human WWOX protein (His tag) (AB86687)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human WWOX protein (His tag) (AB86687)

15% SDS-PAGE showing ab86687 at approximately 28.3kDa (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NZC7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":234,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NZC7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein WWOX also known as WW domain-containing oxidoreductase or FOR has a molecular mass of approximately 46 kDa. Mechanically WWOX functions by interacting with several other proteins through its WW domains impacting various cellular processes. It exhibits a wide expression pattern found in numerous tissues including the brain lung and testis. Additionally WWOX localizes mainly in the cytoplasm but also migrates to the nucleus under certain conditions indicating its diverse roles in cellular regulation.
Biological function summary

The protein plays a significant role in cellular processes like apoptosis tumor suppression and steroidogenesis. WWOX participates in various cellular complexes including ones that regulate transcription and signal transduction. Its interaction with the tumor suppressor p53 and other signaling molecules supports its influence on cell cycle regulation and apoptotic pathways. This interaction aids the cell in sensing stress and damage modulating responses to these conditions.

Pathways

WWOX is intimately linked with the Wnt signaling and apoptotic pathways. In the Wnt pathway WWOX regulates cell proliferation and differentiation by interacting with proteins such as Dvl-2 and beta-catenin. In apoptotic pathways WWOX serves a regulatory role by modulating the activity of proteins like JNK and p73 which are important for inducing programmed cell death. These pathways highlight the importance of WWOX in maintaining cellular homeostasis and responding to abnormal cellular activities.

Alterations in WWOX expression have strong ties to cancer and neurodegenerative diseases. Loss or mutation of WWOX associates with breast prostate and lung cancers due to its role in tumor suppression. The protein also connects to neurodegenerative disorders such as Alzheimer's disease with misregulation affecting neuronal survival. In cancer interactions with proteins like ERBB4 further influence tumorigenic progression while in neurodegeneration WWOX interaction with Tau protein highlights its role in neuron integrity.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques

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General info

Function

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Post-translational modifications

Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.. Ubiquitinated when phosphorylated by TNK2, leading to its degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
See full target information WWOX
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