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AB95931

Recombinant Human WWP1 protein

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Recombinant Human WWP1 protein is a Human Full Length protein, in the 2 to 922 aa range, expressed in Baculovirus, with >15%, suitable for SDS-PAGE.

View Alternative Names

NEDD4-like E3 ubiquitin-protein ligase WWP1, Atrophin-1-interacting protein 5, HECT-type E3 ubiquitin transferase WWP1, TGIF-interacting ubiquitin ligase 1, WW domain-containing protein 1, AIP5, Tiul1, WWP1

1 Images
SDS-PAGE - Recombinant Human WWP1 protein (AB95931)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human WWP1 protein (AB95931)

10% SDS-PAGE showing ab95931 at approximately 106kDa (1μg).

Key facts

Purity

>15% SDS-PAGE

Expression system

Baculovirus

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9H0M0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"106 kDa","actualMolecularWeight":null,"aminoAcidEnd":922,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9H0M0","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WWP1 also known as NEDD4-like E3 ubiquitin-protein ligase operates as an E3 ubiquitin ligase. This protein adds ubiquitin to target substrates marking them for degradation by the proteasome. WWP1 has a molecular mass of approximately 99 kDa. It is expressed in various tissues including the brain heart and skeletal muscles contributing to various cellular processes through its enzymatic activity.
Biological function summary

This protein controls multiple cellular functions by targeting specific proteins for ubiquitination. WWP1 remains an important regulator in cell growth differentiation and immune response. It often acts within protein complexes and associates with multiple substrates and partners. These interactions allow it to control the stability and activity of proteins impacting cellular homeostasis.

Pathways

The ubiquitin-proteasome pathway features WWP1 prominently especially modulating cell cycle regulation and apoptosis. WWP1 also interacts within the TGF-beta signaling pathway impacting cell growth and differentiation. Through these pathways it connects with proteins such as SMAD2/3 and PTEN influencing critical cellular events and signaling processes.

Abnormal WWP1 activity links to cancer and neurodegenerative diseases. In cancer particularly breast and prostate cancer WWP1 can influence tumor growth due to its interactions with proteins like p53 and PTEN. For neurodegenerative diseases impaired WWP1 function affects protein aggregation and degradation contributing to diseases like Alzheimer's where it interacts with amyloid precursor protein (APP).
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. Activates the Hippo signaling pathway in response to cell contact inhibition and recruitment to the Crumbs complex at the cell membrane (PubMed : 34404733). Monoubiquitinates AMOTL2 which facilitates its interaction with and activation of LATS2 (PubMed : 34404733). LATS2 then phosphorylates YAP1, excluding it from the nucleus and therefore ultimately represses YAP1-driven transcription of target genes (PubMed : 34404733).

Post-translational modifications

Auto-ubiquitinated and ubiquitinated by RNF11.

Subcellular localisation

Nucleus

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Product protocols

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. Activates the Hippo signaling pathway in response to cell contact inhibition and recruitment to the Crumbs complex at the cell membrane (PubMed : 34404733). Monoubiquitinates AMOTL2 which facilitates its interaction with and activation of LATS2 (PubMed : 34404733). LATS2 then phosphorylates YAP1, excluding it from the nucleus and therefore ultimately represses YAP1-driven transcription of target genes (PubMed : 34404733).
See full target information WWP1
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