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AB269138

Recombinant human Wwp2 protein (Active)

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Recombinant human Wwp2 protein (Active) is a Human Full Length protein, in the 1 to 870 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

NEDD4-like E3 ubiquitin-protein ligase WWP2, Atrophin-1-interacting protein 2, HECT-type E3 ubiquitin transferase WWP2, WW domain-containing protein 2, AIP2, WWP2

2 Images
Functional Studies - Recombinant human Wwp2 protein (Active) (AB269138)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Wwp2 protein (Active) (AB269138)

The specific activity of ab269138 was 53 nmol/min/mg in an assay using wild-type ubiquitin as substrate.

SDS-PAGE - Recombinant human Wwp2 protein (Active) (AB269138)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Wwp2 protein (Active) (AB269138)

SDS-PAGE analysis of ab269138.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab269138 was 53 nmol/min/mg in an assay using wild-type ubiquitin as substrate.

Accession

O00308

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":870,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O00308","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wwp2 also known as WWP2 or ubiquitin protein ligase E3 component N-terminal homolog 2 is a member of the NEDD4-like E3 ubiquitin ligases. It has a molecular weight of approximately 104 kDa. This protein facilitates the attachment of ubiquitin molecules to substrate proteins tagging them for proteasomal degradation or for regulation of their activity localization or interactions. Wwp2 is expressed in various tissues with higher expression in lung heart muscle and kidney indicating a broad functional range.
Biological function summary

Wwp2 plays a critical role in cellular processes like growth division and differentiation by regulating the stability of key signaling proteins. It functions as part of protein complexes that include WW domain-containing proteins which enables it to interact with multiple substrates. Through its ubiquitination activity Wwp2 influences pathways involved in the maintenance of cellular homeostasis and response to stimuli.

Pathways

Wwp2 has significant involvement in the TGF-beta signaling pathway and the Wnt signaling pathway. In the TGF-beta pathway Wwp2 mediates the degradation of SMAD2 and SMAD7 which are central to the regulation of this signaling cascade. Wwp2 also interacts with proteins like SMURF2 and RNF11 within these pathways promoting fine-tuned control of signal transduction ultimately influencing development and differentiation processes.

Wwp2 is linked to conditions such as cancer and cardiac hypertrophy. Dysregulation of Wwp2 can lead to aberrations in the TGF-beta pathway contributing to oncogenic processes and proliferation of tumor cells. Additionally Wwp2's role in the Wnt pathway associates it with cardiac hypertrophy where altered signaling may disturb cardiac muscle cell growth and function. Noteworthy Wwp2's interaction with proteins like SMAD7 in these contexts offers further insight into its pathological relevance.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

Post-translational modifications

Autoubiquitinated. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome.

Subcellular localisation

Nucleus

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Product protocols

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
See full target information WWP2
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