Recombinant Human XAB1/GPN1 protein is a Human Full Length protein, in the 1 to 374 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M A A S A A A A E L Q A S G G P R H P V C L L V L G M A G S G K T T F V Q R L T G H L H A Q G T P P Y V I N L D P A V H E V P F P A N I D I R D T V K Y K E V M K Q Y G L G P N G G I V T S L N L F A T R F D Q V M K F I E K A Q N M S K Y V L I D T P G Q I E V F T W S A S G T I I T E A L A S S F P T V V I Y V M D T S R S T N P V T F M S N M L Y A C S I L Y K T K L P F I V V M N K T D I I D H S F A V E W M Q D F E A F Q D A L N Q E T T Y V S N L T R S M S L V L D E F Y S S L R V V G V S A V L G T G L D E L F V Q V T S A A E E Y E R E Y R P E Y E R L K K S L A N A E S Q Q Q R E Q L E R L R K D M G S V A L D A G T A K D S L S P V L H P S D L I L T R G T L D E E D E E A D S D T D D I D H R V T E E S H E E P A F Q N F M Q E S M A Q Y W K R N N K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21768307). May act at an RNAP assembly step prior to nuclear import (PubMed:21768307). Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation (PubMed:17643375). May be involved in nuclear localization of XPA (PubMed:11058119).
Alternative names
MBDIN, RPAP4, XAB1, HUSSY-23, GPN1, GPN-loop GTPase 1, MBD2-interacting protein, RNAPII-associated protein 4, XPA-binding protein 1, MBDin
Recommended products
Recombinant Human XAB1/GPN1 protein is a Human Full Length protein, in the 1 to 374 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9HCN4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M A A S A A A A E L Q A S G G P R H P V C L L V L G M A G S G K T T F V Q R L T G H L H A Q G T P P Y V I N L D P A V H E V P F P A N I D I R D T V K Y K E V M K Q Y G L G P N G G I V T S L N L F A T R F D Q V M K F I E K A Q N M S K Y V L I D T P G Q I E V F T W S A S G T I I T E A L A S S F P T V V I Y V M D T S R S T N P V T F M S N M L Y A C S I L Y K T K L P F I V V M N K T D I I D H S F A V E W M Q D F E A F Q D A L N Q E T T Y V S N L T R S M S L V L D E F Y S S L R V V G V S A V L G T G L D E L F V Q V T S A A E E Y E R E Y R P E Y E R L K K S L A N A E S Q Q Q R E Q L E R L R K D M G S V A L D A G T A K D S L S P V L H P S D L I L T R G T L D E E D E E A D S D T D D I D H R V T E E S H E E P A F Q N F M Q E S M A Q Y W K R N N K
- Accession
- Q9HCN4
- Protein length
- Full Length
- Predicted molecular weight
- 44.3 kDa
- Amino acids
- 1 to 374
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab128455 is purified using conventional chromatography techniques.
General info
- Function
Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21768307). May act at an RNAP assembly step prior to nuclear import (PubMed:21768307). Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation (PubMed:17643375). May be involved in nuclear localization of XPA (PubMed:11058119).
- Sequence similarities
Belongs to the GPN-loop GTPase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as XAB1
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XAB1 also known as GPN1 is a small GTPase with an important role in transporting RNA polymerase II from the cytoplasm to the nucleus. XAB1/GPN1 measures around 36 kDa and is expressed ubiquitously in human tissues indicating its importance in cellular functions. It is a member of the GPN-loop GTPase family which shares a conserved domain important for its GTPase activity. The expression patterns suggest it plays a general role in nuclear transport.
- Biological function summary
XAB1/GPN1 contributes to the assembly and transport of RNA polymerase II. It forms a complex with two other GPN-loop GTPases GPN2 and GPN3 which are necessary for RNA polymerase II assembly with other transcription factors and cofactors. The protein’s activity influences how effectively cells can transcribe DNA to RNA a critical step in gene expression. RNA polymerase II plays a significant part in transcribing essential genes including those linked to growth and division.
- Pathways
XAB1/GPN1 integrates into the RNA polymerase II transcription pathway. It also has links with the cell cycle control pathway in collaboration with proteins such as Cyclin-dependent kinases (Cdks) which regulate the cell cycle's progression. GPN1’s activity ensures the efficient transport and functioning of RNA polymerase II and this impacts transcriptional regulation and cell proliferation processes.
- Associated diseases and disorders
XAB1/GPN1 has associations with certain cancer types and neurodegenerative disorders. Abnormalities in its function can lead to disruptions in RNA polymerase II activity potentially contributing to uncontrolled cell growth observed in cancers like glioblastoma. GPN1’s association with faulty gene transcription also affects Huntington's disease implicating interactions with the huntingtin protein. Understanding the role of XAB1/GPN1 in these conditions aids in identifying potential therapeutic targets.
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1 product image
SDS-PAGE - Recombinant Human XAB1/GPN1 protein (ab128455)
15% SDS-PAGE analysis of 3 μg ab128455.
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