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AB128455

Recombinant Human XAB1/GPN1 protein

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Recombinant Human XAB1/GPN1 protein is a Human Full Length protein, in the 1 to 374 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

MBDIN, RPAP4, XAB1, HUSSY-23, GPN1, GPN-loop GTPase 1, MBD2-interacting protein, RNAPII-associated protein 4, XPA-binding protein 1, MBDin

1 Images
SDS-PAGE - Recombinant Human XAB1/GPN1 protein (AB128455)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human XAB1/GPN1 protein (AB128455)

15% SDS-PAGE analysis of 3 μg ab128455.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9HCN4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as XAB1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAASAAAAELQASGGPRHPVCLLVLGMAGSGKTTFVQRLTGHLHAQGTPPYVINLDPAVHEVPFPANIDIRDTVKYKEVMKQYGLGPNGGIVTSLNLFATRFDQVMKFIEKAQNMSKYVLIDTPGQIEVFTWSASGTIITEALASSFPTVVIYVMDTSRSTNPVTFMSNMLYACSILYKTKLPFIVVMNKTDIIDHSFAVEWMQDFEAFQDALNQETTYVSNLTRSMSLVLDEFYSSLRVVGVSAVLGTGLDELFVQVTSAAEEYEREYRPEYERLKKSLANAESQQQREQLERLRKDMGSVALDAGTAKDSLSPVLHPSDLILTRGTLDEEDEEADSDTDDIDHRVTEESHEEPAFQNFMQESMAQYWKRNNK","proteinLength":"Full Length","predictedMolecularWeight":"44.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":374,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9HCN4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XAB1 also known as GPN1 is a small GTPase with an important role in transporting RNA polymerase II from the cytoplasm to the nucleus. XAB1/GPN1 measures around 36 kDa and is expressed ubiquitously in human tissues indicating its importance in cellular functions. It is a member of the GPN-loop GTPase family which shares a conserved domain important for its GTPase activity. The expression patterns suggest it plays a general role in nuclear transport.
Biological function summary

XAB1/GPN1 contributes to the assembly and transport of RNA polymerase II. It forms a complex with two other GPN-loop GTPases GPN2 and GPN3 which are necessary for RNA polymerase II assembly with other transcription factors and cofactors. The protein’s activity influences how effectively cells can transcribe DNA to RNA a critical step in gene expression. RNA polymerase II plays a significant part in transcribing essential genes including those linked to growth and division.

Pathways

XAB1/GPN1 integrates into the RNA polymerase II transcription pathway. It also has links with the cell cycle control pathway in collaboration with proteins such as Cyclin-dependent kinases (Cdks) which regulate the cell cycle's progression. GPN1’s activity ensures the efficient transport and functioning of RNA polymerase II and this impacts transcriptional regulation and cell proliferation processes.

XAB1/GPN1 has associations with certain cancer types and neurodegenerative disorders. Abnormalities in its function can lead to disruptions in RNA polymerase II activity potentially contributing to uncontrolled cell growth observed in cancers like glioblastoma. GPN1’s association with faulty gene transcription also affects Huntington's disease implicating interactions with the huntingtin protein. Understanding the role of XAB1/GPN1 in these conditions aids in identifying potential therapeutic targets.
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Specifications

Form

Liquid

Additional notes

ab128455 is purified using conventional chromatography techniques.

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General info

Function

Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed : 20855544, PubMed : 21768307). May act at an RNAP assembly step prior to nuclear import (PubMed : 21768307). Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation (PubMed : 17643375). May be involved in nuclear localization of XPA (PubMed : 11058119).

Sequence similarities

Belongs to the GPN-loop GTPase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed : 20855544, PubMed : 21768307). May act at an RNAP assembly step prior to nuclear import (PubMed : 21768307). Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation (PubMed : 17643375). May be involved in nuclear localization of XPA (PubMed : 11058119).
See full target information GPN1
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