Recombinant Human XRCC1 protein is a Human Full Length protein, in the 1 to 633 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M P E I R L R H V V S C S S Q D S T H C A E N L L K A D T Y R K W R A A K A G E K T I S V V L Q L E K E E Q I H S V D I G N D G S A F V E V L V G S S A G G A G E Q D Y E V L L V T S S F M S P S E S R S G S N P N R V R M F G P D K L V R A A A E K R W D R V K I V C S Q P Y S K D S P F G L S F V R F H S P P D K D E A E A P S Q K V T V T K L G Q F R V K E E D E S A N S L R P G A L F F S R I N K T S P V T A S D P A G P S Y A A A T L Q A S S A A S S A S P V S R A I G S T S K P Q E S P K G K R K L D L N Q E E K K T P S K P P A Q L S P S V P K R P K L P A P T R T P A T A P V P A R A Q G A V T G K P R G E G T E P R R P R A G P E E L G K I L Q G V V V V L S G F Q N P F R S E L R D K A L E L G A K Y R P D W T R D S T H L I C A F A N T P K Y S Q V L G L G G R I V R K E W V L D C H R M R R R L P S Q R Y L M A G P G S S S E E D E A S H S G G S G D E A P K L P Q K Q P Q T K T K P T Q A A G P S S P Q K P P T P E E T K A A S P V L Q E D I D I E G V Q S E G Q D N G A E D S G D T E D E L R R V A E Q K E H R L P P G Q E E N G E D P Y A G S T D E N T D S E E H Q E P P D L P V P E L P D F F Q G K H F F L Y G E F P G D E R R K L I R Y V T A F N G G L E D Y M S D R V Q F V I T A Q E W D P S F E E A L M D N P S L A F V R P R W I Y S C N E K Q K L L P H Q L Y G V V P Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed:11163244, PubMed:28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed:28002403, PubMed:34102106, PubMed:34811483). Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed:14500814, PubMed:34102106, PubMed:34811483).
Alternative names
DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1
Recommended products
Recombinant Human XRCC1 protein is a Human Full Length protein, in the 1 to 633 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P18887-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P E I R L R H V V S C S S Q D S T H C A E N L L K A D T Y R K W R A A K A G E K T I S V V L Q L E K E E Q I H S V D I G N D G S A F V E V L V G S S A G G A G E Q D Y E V L L V T S S F M S P S E S R S G S N P N R V R M F G P D K L V R A A A E K R W D R V K I V C S Q P Y S K D S P F G L S F V R F H S P P D K D E A E A P S Q K V T V T K L G Q F R V K E E D E S A N S L R P G A L F F S R I N K T S P V T A S D P A G P S Y A A A T L Q A S S A A S S A S P V S R A I G S T S K P Q E S P K G K R K L D L N Q E E K K T P S K P P A Q L S P S V P K R P K L P A P T R T P A T A P V P A R A Q G A V T G K P R G E G T E P R R P R A G P E E L G K I L Q G V V V V L S G F Q N P F R S E L R D K A L E L G A K Y R P D W T R D S T H L I C A F A N T P K Y S Q V L G L G G R I V R K E W V L D C H R M R R R L P S Q R Y L M A G P G S S S E E D E A S H S G G S G D E A P K L P Q K Q P Q T K T K P T Q A A G P S S P Q K P P T P E E T K A A S P V L Q E D I D I E G V Q S E G Q D N G A E D S G D T E D E L R R V A E Q K E H R L P P G Q E E N G E D P Y A G S T D E N T D S E E H Q E P P D L P V P E L P D F F Q G K H F F L Y G E F P G D E R R K L I R Y V T A F N G G L E D Y M S D R V Q F V I T A Q E W D P S F E E A L M D N P S L A F V R P R W I Y S C N E K Q K L L P H Q L Y G V V P Q A
- Accession
- P18887
- Protein length
- Full Length
- Predicted molecular weight
- 95.37 kDa
- Amino acids
- 1 to 633
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed:11163244, PubMed:28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed:28002403, PubMed:34102106, PubMed:34811483). Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed:14500814, PubMed:34102106, PubMed:34811483).
- Post-translational modifications
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XRCC1 (X-ray repair cross-complementing protein 1) plays a role in DNA repair by acting as a scaffold protein. It weighs approximately 70 kDa and is expressed in various tissues reflecting its role in maintaining genomic stability. XRCC1 helps in the repair of single-strand breaks by coordinating the activity of several enzymes involved in the base excision repair pathway. Together with other proteins it helps ensure the integrity of DNA by fostering repair mechanisms following damage by ionizing radiation or other environmental factors.
- Biological function summary
The activity of XRCC1 integrates into DNA damage response as it partners with enzymes like DNA ligase III DNA polymerase beta and poly (ADP-ribose) polymerase (PARP). This protein functions by forming complexes with these enzymes facilitating quick and accurate repair. The ability of XRCC1 to bring together repair machinery is essential for cell survival after DNA damage and plays a role in the prevention of mutagenesis and carcinogenesis as errors in repair processes could lead to genetic mutations.
- Pathways
The XRCC1 protein participates in base excision repair and single-strand break repair pathways. It works closely with PARP1 and DNA ligase III to correct single-strand DNA interruptions. Through these pathways XRCC1 interacts with other repair proteins like TDP1 aiding in the resolution of complex DNA end structures. This collaboration ensures the completion of cellular repair processes maintaining genomic integrity and reducing the likelihood of accumulating harmful mutations.
- Associated diseases and disorders
Mutations in XRCC1 have links to cancer development particularly those involving increased susceptibility to tobacco-related cancers. Studies have connected XRCC1 polymorphisms to a higher risk for conditions such as breast and lung cancer. Relations between XRCC1 and disorders can also be observed with neurodegenerative conditions; for instance alterations in its function or expression patterns might amplify disease progression involving neurological decline. Moreover its interactions with proteins like PARP1 bring forth potential implications in therapeutic strategies aiming to exploit DNA repair pathways for targeted cancer treatments.
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1 product image
SDS-PAGE - Recombinant Human XRCC1 protein (ab131742)
12.5% SDS-PAGE analysis of ab131742, stained with Coomassie Blue.
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