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AB131742

Recombinant Human XRCC1 protein (GST tag N-Terminus)

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Recombinant Human XRCC1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 633 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1

1 Images
SDS-PAGE - Recombinant Human XRCC1 protein (GST tag N-Terminus) (AB131742)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human XRCC1 protein (GST tag N-Terminus) (AB131742)

12.5% SDS-PAGE analysis of ab131742, stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P18887

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB134056

Anti-XRCC1 antibody [EPR4389(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (AB134056)

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Proteins & Peptides

AB159181

Recombinant Human Papillary renal cell carcinoma protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Papillary renal cell carcinoma protein (GST tag N-Terminus) (AB159181)

  • 0
  • 0
Proteins & Peptides

AB80082

Recombinant Human MUC1 protein

SDS-PAGE - Recombinant Human MUC1 protein (AB80082)

  • 0
  • 0
Primary Antibodies

AB109379

Anti-RCC1 antibody [EPR5857]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-RCC1 antibody [EPR5857] (AB109379)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPSYAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLPAPTRTPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGGLEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA","proteinLength":"Full Length","predictedMolecularWeight":"95.37 kDa","actualMolecularWeight":null,"aminoAcidEnd":633,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P18887","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XRCC1 (X-ray repair cross-complementing protein 1) plays a role in DNA repair by acting as a scaffold protein. It weighs approximately 70 kDa and is expressed in various tissues reflecting its role in maintaining genomic stability. XRCC1 helps in the repair of single-strand breaks by coordinating the activity of several enzymes involved in the base excision repair pathway. Together with other proteins it helps ensure the integrity of DNA by fostering repair mechanisms following damage by ionizing radiation or other environmental factors.
Biological function summary

The activity of XRCC1 integrates into DNA damage response as it partners with enzymes like DNA ligase III DNA polymerase beta and poly (ADP-ribose) polymerase (PARP). This protein functions by forming complexes with these enzymes facilitating quick and accurate repair. The ability of XRCC1 to bring together repair machinery is essential for cell survival after DNA damage and plays a role in the prevention of mutagenesis and carcinogenesis as errors in repair processes could lead to genetic mutations.

Pathways

The XRCC1 protein participates in base excision repair and single-strand break repair pathways. It works closely with PARP1 and DNA ligase III to correct single-strand DNA interruptions. Through these pathways XRCC1 interacts with other repair proteins like TDP1 aiding in the resolution of complex DNA end structures. This collaboration ensures the completion of cellular repair processes maintaining genomic integrity and reducing the likelihood of accumulating harmful mutations.

Mutations in XRCC1 have links to cancer development particularly those involving increased susceptibility to tobacco-related cancers. Studies have connected XRCC1 polymorphisms to a higher risk for conditions such as breast and lung cancer. Relations between XRCC1 and disorders can also be observed with neurodegenerative conditions; for instance alterations in its function or expression patterns might amplify disease progression involving neurological decline. Moreover its interactions with proteins like PARP1 bring forth potential implications in therapeutic strategies aiming to exploit DNA repair pathways for targeted cancer treatments.
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Specifications

Form

Liquid

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General info

Function

Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed : 11163244, PubMed : 28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed : 28002403, PubMed : 34102106, PubMed : 34811483). Recognizes and binds poly-ADP-ribose chains : specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed : 14500814, PubMed : 34102106, PubMed : 34811483).

Post-translational modifications

Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.. Sumoylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed : 11163244, PubMed : 28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed : 28002403, PubMed : 34102106, PubMed : 34811483). Recognizes and binds poly-ADP-ribose chains : specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed : 14500814, PubMed : 34102106, PubMed : 34811483).
See full target information XRCC1
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