Recombinant Human XRCC2 protein (denatured) is a Human Full Length protein, in the 1 to 280 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M C S A F H R A E S G T E L L A R L E G R S S L K E I E P N L F A D E D S P V H G D I L E F H G P E G T G K T E M L Y H L T A R C I L P K S E G G L E V E V L F I D T D Y H F D M L R L V T I L E H R L S Q S S E E I I K Y C L G R F F L V Y C S S S T H L L L T L Y S L E S M F C S H P S L C L L I L D S L S A F Y W I D R V N G G E S V N L Q E S T L R K C S Q C L E K L V N D Y R L V L F A T T Q T I M Q K A S S S S E E P S H A S R R L C D V D I D Y R P Y L C K A W Q Q L V K H R M F F S K Q D D S Q S S N Q F S L V S R C L K S N S L K K H F F I I G E S G V E F C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Alternative names
DNA repair protein XRCC2, X-ray repair cross-complementing protein 2, XRCC2
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Recombinant Human XRCC2 protein (denatured) is a Human Full Length protein, in the 1 to 280 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O43543-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M C S A F H R A E S G T E L L A R L E G R S S L K E I E P N L F A D E D S P V H G D I L E F H G P E G T G K T E M L Y H L T A R C I L P K S E G G L E V E V L F I D T D Y H F D M L R L V T I L E H R L S Q S S E E I I K Y C L G R F F L V Y C S S S T H L L L T L Y S L E S M F C S H P S L C L L I L D S L S A F Y W I D R V N G G E S V N L Q E S T L R K C S Q C L E K L V N D Y R L V L F A T T Q T I M Q K A S S S S E E P S H A S R R L C D V D I D Y R P Y L C K A W Q Q L V K H R M F F S K Q D D S Q S S N Q F S L V S R C L K S N S L K K H F F I I G E S G V E F C
- Accession
- O43543
- Protein length
- Full Length
- Predicted molecular weight
- 34.3 kDa
- Amino acids
- 1 to 280
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
- Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XRCC2 also known as X-ray repair cross-complementing protein 2 is a protein that plays a role in DNA repair mechanisms. It has a molecular mass of approximately 33 kDa. XRCC2 is expressed in various tissues with noticeable abundance in proliferative tissues due to their high demand for DNA repair processes. Mechanistically XRCC2 functions as part of the Rad51 paralog group facilitating homologous recombination repair of DNA double-strand breaks. Its involvement is quintessential for maintaining genomic stability.
- Biological function summary
XRCC2 contributes to cellular maintenance by repairing DNA double-strand breaks through the homologous recombination pathway. It participates in the Rad51 paralog complex alongside other paralogs like Rad51B Rad51C Rad51D and XRCC3. This complex ensures precise repair and re-ligation of damaged DNA by assisting in strand exchange and stabilization. Such actions highlight XRCC2's role in preserving cell genetic integrity and preventing mutations.
- Pathways
Homologous recombination and DNA repair are integral processes where XRCC2 operates. The homologous recombination pathway is critically linked with the maintenance of chromosome integrity during cell division. XRCC2 by cooperating with proteins like RAD51 and BRCA2 forms an essential part of recombinational DNA repair. Its interactions are necessary for orchestrating the repair process ensuring seamless recovery of the genetic code after disruptions.
- Associated diseases and disorders
XRCC2 is associated closely with cancer vulnerabilities due to its role in DNA repair. Deficiencies or mutations in XRCC2 can lead to increased susceptibility to tumors and hereditary breast cancer given its involvement in stabilizing RAD51 function which is pivotal in suppressing tumor formation. The malfunction in XRCC2's DNA repair capabilities can also have connections with Fanconi anemia highlighting XRCC2’s importance to genetic disorders related to impaired DNA repair mechanisms.
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1 product image
SDS-PAGE - Recombinant Human XRCC2 protein (denatured) (ab177641)
15% SDS-PAGE analysis of ab177641 (3ug)
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