Recombinant Human YAP1 protein (His tag) is a Human Full Length protein, in the 1 to 504 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M D P G Q Q P P P Q P A P Q G Q G Q P P S Q P P Q G Q G P P S G P G Q P A P A A T Q A A P Q A P P A G H Q I V H V R G D S E T D L E A L F N A V M N P K T A N V P Q T V P M R L R K L P D S F F K P P E P K S H S R Q A S T D A G T A G A L T P Q H V R A H S S P A S L Q L G A V S P G T L T P T G V V S G P A A T P T A Q H L R Q S S F E I P D D V P L P A G W E M A K T S S G Q R Y F L N H I D Q T T T W Q D P R K A M L S Q M N V T A P T S P P V Q Q N M M N S A S G P L P D G W E Q A M T Q D G E I Y Y I N H K N K T T S W L D P R L D P R F A M N Q R I S Q S A P V K Q P P P L A P Q S P Q G G V M G G S N S N Q Q Q Q M R L Q Q L Q M E K E R L R L K Q Q E L L R Q A M R N I N P S T A N S P K C Q E L A L R S Q L P T L E Q D G G T Q N P V S S P G M S Q E L R T M T T N S S D P F L N S G T Y H S R D E S T D S G L S M S S Y S V P R T P D D F L N S V D E M D T G D T I N Q S T L P S Q Q N R F P D Y L E A I P G T N V D L G T L E G D G M N I E G E E L M P S L Q E A L S S D I L N D M E S V L A A T K L D K E S F L T W L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Phosphorylation of YAP1 by LATS1/2 prevents its nuclear translocation, thereby regulating the expression of its target genes (PubMed:18158288, PubMed:26598551, PubMed:34404733). The transcriptional regulation of gene expression requires TEAD transcription factors and modulates cell growth, anchorage-independent growth, and induction of epithelial-mesenchymal transition (EMT) (PubMed:18579750). Plays a key role in tissue tension and 3D tissue shape by regulating the cortical actomyosin network, acting via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). It also suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Synergizes with WBP2 to enhance PGR activity (PubMed:16772533). Isoform 2. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Isoform 3. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Alternative names
YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog
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Recombinant Human YAP1 protein (His tag) is a Human Full Length protein, in the 1 to 504 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P46937-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M D P G Q Q P P P Q P A P Q G Q G Q P P S Q P P Q G Q G P P S G P G Q P A P A A T Q A A P Q A P P A G H Q I V H V R G D S E T D L E A L F N A V M N P K T A N V P Q T V P M R L R K L P D S F F K P P E P K S H S R Q A S T D A G T A G A L T P Q H V R A H S S P A S L Q L G A V S P G T L T P T G V V S G P A A T P T A Q H L R Q S S F E I P D D V P L P A G W E M A K T S S G Q R Y F L N H I D Q T T T W Q D P R K A M L S Q M N V T A P T S P P V Q Q N M M N S A S G P L P D G W E Q A M T Q D G E I Y Y I N H K N K T T S W L D P R L D P R F A M N Q R I S Q S A P V K Q P P P L A P Q S P Q G G V M G G S N S N Q Q Q Q M R L Q Q L Q M E K E R L R L K Q Q E L L R Q A M R N I N P S T A N S P K C Q E L A L R S Q L P T L E Q D G G T Q N P V S S P G M S Q E L R T M T T N S S D P F L N S G T Y H S R D E S T D S G L S M S S Y S V P R T P D D F L N S V D E M D T G D T I N Q S T L P S Q Q N R F P D Y L E A I P G T N V D L G T L E G D G M N I E G E E L M P S L Q E A L S S D I L N D M E S V L A A T K L D K E S F L T W L
- Accession
- P46937
- Protein length
- Full Length
- Predicted molecular weight
- 56.5 kDa
- Amino acids
- 1 to 504
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Phosphorylation of YAP1 by LATS1/2 prevents its nuclear translocation, thereby regulating the expression of its target genes (PubMed:18158288, PubMed:26598551, PubMed:34404733). The transcriptional regulation of gene expression requires TEAD transcription factors and modulates cell growth, anchorage-independent growth, and induction of epithelial-mesenchymal transition (EMT) (PubMed:18579750). Plays a key role in tissue tension and 3D tissue shape by regulating the cortical actomyosin network, acting via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). It also suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Synergizes with WBP2 to enhance PGR activity (PubMed:16772533).
- Sequence similarities
Belongs to the YAP1 family.
- Post-translational modifications
Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation (PubMed:18158288, PubMed:20048001). Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage (PubMed:18280240). Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation (PubMed:20048001). Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1 (PubMed:21364637). Phosphorylated in the nucleus by PRP4K; phosphorylation leads to nuclear exclusion (PubMed:29695716).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.
- Biological function summary
YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.
- Pathways
YAP1 is importantly involved in the Hippo signaling pathway and Wnt/β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/β-catenin pathway YAP1 interacts with β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.
- Associated diseases and disorders
YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.
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1 product image
SDS-PAGE - Recombinant Human YAP1 protein (His tag) (ab241236)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab241236.
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