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AB126680

Recombinant Human YARS2/TyRS protein (His tag N-Terminus)

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Recombinant Human YARS2/TyRS protein (His tag N-Terminus) is a Human Full Length protein, in the 17 to 477 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CGI-04, YARS2, Tyrosyl-tRNA synthetase, TyrRS

1 Images
SDS-PAGE - Recombinant Human YARS2/TyRS protein (His tag N-Terminus) (AB126680)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human YARS2/TyRS protein (His tag N-Terminus) (AB126680)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y2Z4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein previously labeled as YARS2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMTLNLSVLLPLGLRKAHSGAQGLLAAQKARGLFKDFFPETGTKIELPELFDRGTASFPQTIYCGFDPTADSLHVGHLLALLGLFHLQRAGHNVIALVGGATARLGDPSGRTKEREALETERVRANARALRLGLEALAANHQQLFTDGRSWGSFTVLDNSAWYQKQHLVDFLAAVGGHFRMGTLLSRQSVQLRLKSPEGMSLAEFFYQVLQAYDFYYLFQRYGCRVQLGGSDQLGNIMSGYEFINKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNRDKTSPFELYQFFVRQPDDSVERYLKLFTFLPLPEIDHIMQLHVKEPERRGPQKRLAAEVTKLVHGREGLDSAKRCTQALYHSSIDALEVMSDQELKELFKEAPFSEFFLDPGTSVLDTCRKANAIPDGPRGYRMITEGGVSINHQQVTNPESVLIVGQHILKNGLSLLKIGKRNFYIIKWLQL","proteinLength":"Full Length","predictedMolecularWeight":"53.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":477,"aminoAcidStart":17,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y2Z4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YARS2 also known as TyrRS is a mitochondrial tyrosyl-tRNA synthetase with a molecular mass of approximately 55 kDa. It catalyzes the attachment of tyrosine to its corresponding tRNA a fundamental step for protein synthesis within mitochondria. YARS2 is expressed in various tissues with notable levels in those requiring high mitochondrial activity. It ensures the correct translation of mitochondrial genes by ensuring the specific aminoacylation of tRNA.
Biological function summary

YARS2 plays an essential role in mitochondrial protein biosynthesis a process vital for cellular energy production. As part of the group of aminoacyl-tRNA synthetases it is required for proper mitochondrial function and cellular respiration. Mitochondrial dysfunction due to errors in this enzyme can disrupt cellular homeostasis and energy metabolism leading to severe outcomes.

Pathways

The activity of YARS2 is integral to the oxidative phosphorylation pathway. This pathway involves the production of ATP the energy currency of the cell and requires a coordinated action of multiple proteins including cytochrome c oxidase subunits. YARS2 ensures the synthesis of key proteins necessary for this pathway influencing both energy generation and overall cellular health.

YARS2 mutations relate to mitochondrial myopathy and sideroblastic anemia. These conditions often involve the disruption of normal mitochondrial protein synthesis leading to muscle weakness and compromised red blood cell production. Mutations in related proteins such as MTO1 and TRMU which also influence mitochondrial translation can exacerbate these disorders highlighting the critical role of YARS2 within mitochondrial genetic translation and its clinical significance.
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Specifications

Form

Liquid

Additional notes

ab126680 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction : tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction : tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
See full target information YARS2
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Complementary Products

Primary Antibodies

AB249125

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9927] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9927] - BSA and Azide free (AB249125)

  • 0
  • 0
Primary Antibodies

AB228957

Anti-YARS2/TyRS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YARS2/TyRS antibody (AB228957)

  • 0
  • 0
Primary Antibodies

AB111697

Anti-ZNF587 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF587 antibody (AB111697)

  • 0
  • 0
Primary Antibodies

AB198171

Anti-NOC2L antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOC2L antibody (AB198171)

  • 0
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