Recombinant human Yes1 protein (Active)
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Recombinant human Yes1 protein (Active) is a Human Full Length protein, in the 2 to 543 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
YES, YES1, Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes
- FuncS
Unknown
Functional Studies - Recombinant human Yes1 protein (Active) (AB101504)
The specific activity of Yes1 (ab101504) was determined to be 173.2 nmol/min/mg as per activity assay protocol
- FuncS
Unknown
Functional Studies - Recombinant human Yes1 protein (Active) (AB101504)
The Specific activity of ab101504 was determined to be 185 nmol/min/mg.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human Yes1 protein (Active) (AB101504)
SDS PAGE analysis of ab101504
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human Yes1 protein (Active) (AB101504)
SDS-PAGE analysis of ab101504.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.
Pathways
The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.
Specifications
Form
Liquid
Additional notes
The purity was determined to be >80% by densitometry. Affinity purified.
General info
Function
Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed : 26979622).
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Post-translational modifications
Phosphorylated (PubMed:11901164). Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-426 maintains enzyme activity by blocking CSK-mediated inhibition.. Palmitoylation at Cys-3 promotes membrane localization.
Subcellular localisation
Cytoskeleton
Target data
Complementary Products
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Product promise
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