Recombinant Human YKL-40/CHI3L1 protein is a Human Full Length protein, in the 22 to 383 aa range, expressed in CHO, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- CHO cells
- Tags
- His tag C-Terminus Myc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
Y K L V C Y Y T S W S Q Y R E G D G S C F P D A L D R F L C T H I I Y S F A N I S N D H I D T W E W N D V T L Y G M L N T L K N R N P N L K T L L S V G G W N F G S Q R F S K I A S N T Q S R R T F I K S V P P F L R T H G F D G L D L A W L Y P G R R D K Q H F T T L I K E M K A E F I K E A Q P G K K Q L L L S A A L S A G K V T I D S S Y D I A K I S Q H L D F I S I M T Y D F H G A W R G T T G H H S P L F R G Q E D A S P D R F S N T D Y A V G Y M L R L G A P A S K L V M G I P T F G R S F T L A S S E T G V G A P I S G P G I P G R F T K E A G T L A Y Y E I C D F L R G A T V H R I L G Q Q V P Y A T K G N Q W V G Y D D Q E S V K S K V Q Y L K D R Q L A G A M V W A L D L D D F Q G S F C G Q D L R F P L T N A I K D A L A A T K L G P E Q K L I S E E D L N S A V D H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Alternative names
Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1
Recommended products
Recombinant Human YKL-40/CHI3L1 protein is a Human Full Length protein, in the 22 to 383 aa range, expressed in CHO, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- CHO cells
- Tags
- His tag C-Terminus Myc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P36222-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% PBS
Sequence info
Amino acid sequence
- Y K L V C Y Y T S W S Q Y R E G D G S C F P D A L D R F L C T H I I Y S F A N I S N D H I D T W E W N D V T L Y G M L N T L K N R N P N L K T L L S V G G W N F G S Q R F S K I A S N T Q S R R T F I K S V P P F L R T H G F D G L D L A W L Y P G R R D K Q H F T T L I K E M K A E F I K E A Q P G K K Q L L L S A A L S A G K V T I D S S Y D I A K I S Q H L D F I S I M T Y D F H G A W R G T T G H H S P L F R G Q E D A S P D R F S N T D Y A V G Y M L R L G A P A S K L V M G I P T F G R S F T L A S S E T G V G A P I S G P G I P G R F T K E A G T L A Y Y E I C D F L R G A T V H R I L G Q Q V P Y A T K G N Q W V G Y D D Q E S V K S K V Q Y L K D R Q L A G A M V W A L D L D D F Q G S F C G Q D L R F P L T N A I K D A L A A T K L G P E Q K L I S E E D L N S A V D H H H H H H
- Accession
- P36222
- Protein length
- Full Length
- Predicted molecular weight
- 43.3 kDa
- Amino acids
- 22 to 383
- Nature
- Recombinant
- Tags
- His tag C-Terminus, Myc tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab140057 was purified by using conventional chromatography techniques.
General info
- Function
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
- Sequence similarities
Belongs to the glycosyl hydrolase 18 family.
- Post-translational modifications
Glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as CHI3L1
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.
- Biological function summary
YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.
- Pathways
YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.
- Associated diseases and disorders
YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.
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SDS-PAGE - Recombinant Human YKL-40/CHI3L1 protein (ab140057)
15% SDS-PAGE analysis of ab140057 (3µg).
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