Recombinant human YKL-40/CHI3L1 protein

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 50000 cells per well are added to rhCHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner.
The ED₅₀ for this effect is typically 0.8 – 3.5 μg/ml.

Predicted molecular weight

41.3 kDa

Amino acids

22 to 383

Sequence

Y K L V C Y Y T S W S Q Y R E G D G S C F P D A L D R F L C T H I I Y S F A N I S N D H I D T W E W N D V T L Y G M L N

T L K N R N P N L K T L L S V G G W N F G S Q R F S K I A S N T Q S R R T F I K S V P P F L R T H G F D G L D L A W L Y

P G R R D K Q H F T T L I K E M K A E F I K E A Q P G K K Q L L L S A A L S A G K V T I D S S Y D I A K I S Q H L D F I

S I M T Y D F H G A W R G T T G H H S P L F R G Q E D A S P D R F S N T D Y A V G Y M L R L G A P A S K L V M G I P T F

G R S F T L A S S E T G V G A P I S G P G I P G R F T K E A G T L A Y Y E I C D F L R G A T V H R I L G Q Q V P Y A T K

G N Q W V G Y D D Q E S V K S K V Q Y L K D R Q L A G A M V W A L D L D D F Q G S F C G Q D L R F P L T N A I K D A L A

A T

Accession

P36222-1

Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Concentration

Storage buffer

pH: 7.4
Constituents: 95% PBS, 5% Trehalose

Notes

Product promise